Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4.
Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4.
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DOI：
10.1091/mbc.e10-01-0074
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发表时间：
2010-08-01
影响因子：
3.3
通讯作者：
Shin HW
中科院分区：
文献类型：
--
作者：
Yamamoto H;Koga H;Katoh Y;Takahashi S;Nakayama K;Shin HW
Rab 14以GTP依赖性方式与RUFY 1/Rabip 4结合，RUFY 1/Rabip 4最初被鉴定为Rab 4效应子。我们认为Rab 14和Rab 4顺序作用; Rab 14是RUFY 1募集到内体上所必需的，随后RUFY 1与Rab 4的相互作用可能允许内体束缚和融合。小的GTribRab 14定位于早期内体和trans-Golgi网络，但其在内体上的细胞功能及其与其他内体Rab蛋白的功能关系知之甚少。在这里，我们报告Rab 14以GTP依赖性方式结合RUFY 1/Rabip 4，RUFY 1/Rabip 4最初被鉴定为Rab 4效应子。Rab 14与Rab 4在外周核内体上共定位良好。Rab 14的缺失，而不是Rab 4，导致RUFY 1从内体膜上解离。RUFY 1与Rab 14或Rab 4的共表达诱导内体的聚集和扩大，而缺乏Rab 4结合区域的RUFY 1突变体即使与Rab 14或Rab 4共表达也不会诱导内体结构的显著形态学变化。这些发现表明Rab 14和Rab 4与RUFY 1一起顺序作用; Rab 14是将RUFY 1募集到内体膜上所必需的，随后RUFY 1与Rab 4的相互作用可能允许内体束缚和融合。Rab 14或RUFY 1以及Rab 4的耗竭抑制内吞转铁蛋白的有效再循环，表明Rab 14和Rab 4通过与其双重效应子RUFY 1的协同相互作用来调节内体功能。
Rab14 binds in a GTP-dependent manner to RUFY1/Rabip4, which had been originally identified as a Rab4 effector. We suggest that Rab14 and Rab4 act sequentially; Rab14 is required for recruitment of RUFY1 onto endosomes and subsequent RUFY1 interaction with Rab4 may allow endosomal tethering and fusion. The small GTPase Rab14 localizes to early endosomes and the trans-Golgi network, but its cellular functions on endosomes and its functional relationship with other endosomal Rab proteins are poorly understood. Here, we report that Rab14 binds in a GTP-dependent manner to RUFY1/Rabip4, which had been originally identified as a Rab4 effector. Rab14 colocalizes well with Rab4 on peripheral endosomes. Depletion of Rab14, but not Rab4, causes dissociation of RUFY1 from endosomal membranes. Coexpression of RUFY1 with either Rab14 or Rab4 induces clustering and enlargement of endosomes, whereas a RUFY1 mutant lacking the Rab4-binding region does not induce a significant morphological change in the endosomal structures even when coexpressed with Rab14 or Rab4. These findings suggest that Rab14 and Rab4 act sequentially, together with RUFY1; Rab14 is required for recruitment of RUFY1 onto endosomal membranes, and subsequent RUFY1 interaction with Rab4 may allow endosomal tethering and fusion. Depletion of Rab14 or RUFY1, as well as Rab4, inhibits efficient recycling of endocytosed transferrin, suggesting that Rab14 and Rab4 regulate endosomal functions through cooperative interactions with their dual effector, RUFY1.
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影响因子：
64.5
作者：
Horiuchi, H;Lippe, R;Zerial, M
通讯作者：
Zerial, M
影响因子：
4.8
作者：
Lindsay, AJ;Hendrick, AG;McCaffrey, MW
通讯作者：
McCaffrey, MW
DOI：
10.1083/jcb.131.6.1435
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Feng Y;Press B;Wandinger-Ness A
通讯作者：
Wandinger-Ness A
影响因子：
11.4
作者：
Kyei, George B.;Vergne, Isabelle;Deretic, Vojo
通讯作者：
Deretic, Vojo
影响因子：
4.1
作者：
Nagelkerken, B;van Anken, E;van der Sluijs, P
通讯作者：
van der Sluijs, P