Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling.
Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling.
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脱落酸信号传导中丝裂原激活蛋白激酶级联对玉米 NADPH 氧化酶的正反馈调节
DOI：
10.1093/jxb/erp157
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发表时间：
2009
影响因子：
6.9
通讯作者：
Jiang M
中科院分区：
文献类型：
--
作者：
Lin F;Ding H;Wang J;Zhang H;Zhang A;Zhang Y;Tan M;Dong W;Jiang M
在玉米(Zea Mays)中，脱落酸(ABA)诱导的过氧化氢的产生激活了46 kDa的丝裂原激活的蛋白激酶(P46MAPK)，而p46MAPK的激活也调节了过氧化氢的产生。然而，MAPK在ABA信号转导中调节过氧化氢产生的机制仍有待阐明。本研究从玉米叶片中分离并鉴定了4个产生活性氧(ROS)的NADPH氧化酶(Rboh)基因(ZmrbohA-D)。ABA处理诱导ZmrbohA-D的表达和NADPH氧化酶活性的双相反应(I期和II期)。两种丝裂原活化蛋白激酶(MPKKK)抑制剂和两种H_2O_2清除剂可阻断ABA诱导的细胞周期II期，但对I期无明显影响。单独用H_2O_2处理也只诱导了细胞周期II期，这种诱导作用被MAPKK抑制剂阻断。此外，对ABA激活的p46MAPK进行了部分纯化。根据p46MAPK基因序列设计了相应的内切酶序列，克隆了p46MAPK基因。对胰酶多肽和p46MAPK序列的分析表明，它就是已知的ZmMPK5。ABA和H_2O_2处理显著提高了ZmMPK5的活性，而ABA仅诱导了ZmMPK5的表达略有增加。结果表明，过氧化氢激活的ZmMPK5参与了ABA信号转导中II相的激活，但不参与I相的激活。结果表明，ABA信号转导中存在NADPH氧化酶、H_2O_2和ZmMPK5正反馈回路。
In maize (Zea mays), abscisic acid (ABA)-induced H2O2production activates a 46 kDa mitogen-activated protein kinase (p46MAPK), and the activation of p46MAPK also regulates the production of H2O2. However, the mechanism for the regulation of H2O2production by MAPK in ABA signalling remains to be elucidated. In this study, four reactive oxygen species (ROS)-producing NADPH oxidase (rboh) genes (ZmrbohA–D) were isolated and characterized in maize leaves. ABA treatment induced a biphasic response (phase I and phase II) in the expression ofZmrbohA–Dand the activity of NADPH oxidase. Phase II induced by ABA was blocked by pretreatments with two MAPK kinase (MPKKK) inhibitors and two H2O2scavengers, but phase I was not affected by these inhibitors or scavengers. Treatment with H2O2alone also only induced phase II, and the induction was arrested by the MAPKK inhibitors. Furthermore, the ABA-activated p46MAPK was partially purified. Using primers corresponding to the sequences of internal tryptic peptides, thep46MAPKgene was cloned. Analysis of the tryptic peptides and the p46MAPK sequence indicate it is the known ZmMPK5. Treatments with ABA and H2O2led to a significant increase in the activity of ZmMPK5, although ABA treatment only induced a slight increase in the expression ofZmMPK5. The data indicate that H2O2-activated ZmMPK5 is involved in the activation of phase II in ABA signalling, but not in phase I. The results suggest that there is a positive feedback loop involving NADPH oxidase, H2O2, and ZmMPK5 in ABA signalling.
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影响因子：
7.4
作者：
Kwak, JM;Nguyen, V;Schroeder, JI
通讯作者：
Schroeder, JI
影响因子：
6.9
作者：
Jiang, MY;Zhang, JH
通讯作者：
Zhang, JH
影响因子：
6.9
作者：
Burnett, EC;Desikan, R;Neill, SJ
通讯作者：
Neill, SJ
影响因子：
7.2
作者：
Groom, QJ;Torres, MA;Jones, JDG
通讯作者：
Jones, JDG
DOI：
10.1073/pnas.97.6.2940
发表时间：
2000-03-14
影响因子：
11.1
作者：
Kovtun, Y;Chiu, WL;Sheen, J
通讯作者：
Sheen, J