Mutation rate plasticity in rifampicin resistance depends on Escherichia coli cell-cell interactions.
Mutation rate plasticity in rifampicin resistance depends on Escherichia coli cell-cell interactions.
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DOI：
10.1038/ncomms4742
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发表时间：
2014-04-29
影响因子：
16.6
通讯作者：
Knight, Christopher G.
中科院分区：
文献类型：
--
作者：
Krasovec, Rok;Belavkin, Roman V.;Aston, John A. D.;Channon, Alastair;Aston, Elizabeth;Rash, Bharat M.;Kadirvel, Manikandan;Forbes, Sarah;Knight, Christopher G.
特定基因型中特定位点的突变率变化，换句话说，突变率可塑性（MRP），可以由压力或老化引起。然而，突变率的控制由其他因素是不太好的特点。在这里，我们表明，在野生型大肠杆菌（K-12和B菌株），利福平耐药的突变率是塑料和人口密度呈负相关：降低密度可以增加突变率至少三倍。这种MRP是遗传上可转换的，依赖于群体感应基因luxS-特别是它在活化甲基循环中的作用-并且通过细胞-细胞相互作用进行社会介导。虽然我们确定在某些情况下，突变率与健身的负相关，我们没有发现功能与应力诱导的诱变。我们通过社会环境对突变率的实验性操纵提高了这种操纵发生在自然界中并可以在医学上利用的可能性。 在单一基因型中改变特定位点突变率的因素仍然难以捉摸。在这里，Kraškovic等人表明，大肠杆菌中利福平耐药位点的突变率随着种群密度的增加而降低，并且突变率的可塑性是由luxS基因控制的。
Variation of mutation rate at a particular site in a particular genotype, in other words mutation rate plasticity (MRP), can be caused by stress or ageing. However, mutation rate control by other factors is less well characterized. Here we show that in wild-type Escherichia coli (K-12 and B strains), the mutation rate to rifampicin resistance is plastic and inversely related to population density: lowering density can increase mutation rates at least threefold. This MRP is genetically switchable, dependent on the quorum-sensing gene luxS—specifically its role in the activated methyl cycle—and is socially mediated via cell–cell interactions. Although we identify an inverse association of mutation rate with fitness under some circumstances, we find no functional link with stress-induced mutagenesis. Our experimental manipulation of mutation rates via the social environment raises the possibility that such manipulation occurs in nature and could be exploited medically. The factors varying mutation rate at a particular site in a single genotype remain elusive. Here, Krašovec et al. show that mutation rates at sites conferring resistance to rifampicin in Escherichia coli decrease with population density, and that mutation-rate plasticity is controlled by the luxS gene.
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DOI：
10.1073/pnas.1210309109
发表时间：
2012-10-09
影响因子：
11.1
作者：
Lee, Heewook;Popodi, Ellen;Foster, Patricia L.
通讯作者：
Foster, Patricia L.
影响因子：
2.9
作者：
LENSKI, RE;ROSE, MR;TADLER, SC
通讯作者：
TADLER, SC
DOI：
10.1017/s0016672300010284
发表时间：
1967-01-01
期刊：
GENETICAL RESEARCH
影响因子：
--
作者：
KIMURA, M
通讯作者：
KIMURA, M
影响因子：
64.8
作者：
COULONDRE, C;MILLER, JH;GILBERT, W
通讯作者：
GILBERT, W
影响因子：
64.8
作者：
Cooper, VS;Lenski, RE
通讯作者：
Lenski, RE