A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain: use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach.
A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain: use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach.
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DOI：
10.1007/s10120-011-0125-2
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发表时间：
2012-10
期刊：
影响因子：
7.4
通讯作者：
Goto, Masamichi
中科院分区：
文献类型：
--
作者：
Yonezawa, Suguru;Kitajima, Shinichi;Higashi, Michiyo;Osako, Masahiko;Horinouchi, Michiko;Yokoyama, Seiya;Kitamoto, Sho;Yamada, Norishige;Tamura, Yukihiro;Shimizu, Takeshi;Tabata, Mineo;Goto, Masamichi
非实体型低分化腺癌（por 2）或印戒细胞癌（sig）的孤立癌细胞常见于硬癌，预后极差。这些细胞常散在分布于肉芽组织或结缔组织增生性纤维化组织中，在常规病理检查中易被忽视。我们的目的是提出一种新的抗体，可以很容易地识别分离的癌细胞。由于MUC 1胞质尾区（CTD）具有多种生物学作用，包括肿瘤进展和细胞粘附障碍，并且预期在分离的癌细胞中表达，因此我们提出了针对胞内非重复的19个氨基酸序列的新型单克隆抗体（MAb）MUC 1 - 014 E（RYVPPSSTDRSPYEKVSAG：N-1217-1235-C），使用包括7-氨基酸表位（STDRSPY：N-1223-1229-C）的合成肽。在107例胃切除标本（包括48例por 2和31例sig病变）的免疫组化染色中，MAb MUC 1 - 014 E对por 2（100%）和sig（97%）显示出高阳性率（≥5%的癌细胞染色），对por 2（100%）和sig（90%）显示出最高强度的染色（4+，≥75%的癌细胞染色）。在包括82个por 2和38个sig病变的89个活检标本中，MAb MUC 1 - 014 E显示por 2（100%）和sig（100%）的高阳性染色率以及por 2（87%）和sig（84%）的4+染色率。与细胞角蛋白（AE 1/AE 3或CAM 5.2）组相比，差异有显著性（P <0.05）。MUC 1 - 014 E单克隆抗体对于准确检测硬癌中的分离癌细胞非常有用。
Isolated cancer cells of non-solid type poorly differentiated adenocarcinoma (por2) or signet-ring cell carcinoma (sig) are frequently seen in scirrhous gastric cancers with a very poor prognosis. These cells are often scattered in granulation tissue or desmoplastic fibrotic tissue and tend to be overlooked in routine pathological examination. We aimed to raise a novel antibody that can identify the isolated cancer cells easily. Because the MUC1 cytoplasmic tail domain (CTD) has many biological roles including tumor progression and cell adhesion disturbance and is expected to be expressed in isolated cancer cells, we raised a novel monoclonal antibody (MAb) MUC1-014E against an intracellular nonrepeating 19-amino-acid sequence (RYVPPSSTDRSPYEKVSAG: N-1217-1235-C) of the MUC1 CTD, using a synthetic peptide including the 7-amino-acid epitope (STDRSPY: N-1223-1229-C). In the immunohistochemical staining of 107 gastrectomy specimens including 48 por2 and 31 sig lesions, the MAb MUC1-014E showed high rates of positive staining (≥5% of carcinoma cells stained) for por2 (100%) and sig (97%), and of the highest intensity staining (4+, ≥75% of carcinoma cells stained) for por2 (100%) and sig (90%). In the 89 biopsy specimens including 82 por2 and 38 sig lesions, the MAb MUC1-014E showed high rates of positive staining for por2 (100%) and sig (100%) and of 4+ staining for por2 (87%) and sig (84%). All the rates were significantly higher than those with cytokeratins (AE1/AE3 or CAM5.2). The MAb MUC1-014E is very useful for accurate detection of isolated cancer cells in scirrhous gastric cancers.
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影响因子：
5.2
作者：
Ren, Jian;Raina, Deepak;Kufe, Donald
通讯作者：
Kufe, Donald
DOI：
10.1073/pnas.85.7.2320
发表时间：
1988-04-01
影响因子：
11.1
作者：
SIDDIQUI, J;ABE, M;KUFE, D
通讯作者：
KUFE, D
影响因子：
78.5
作者：
Kufe, Donald W.
通讯作者：
Kufe, Donald W.
影响因子：
3.7
作者：
Mahanta S;Fessler SP;Park J;Bamdad C
通讯作者：
Bamdad C
影响因子：
7.3
作者：
Carson, Daniel D.
通讯作者：
Carson, Daniel D.