Reduced expression of PinX1 correlates to progressive features in patients with prostate cancer.
Reduced expression of PinX1 correlates to progressive features in patients with prostate cancer.
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PinX1 表达减少与前列腺癌患者的进展特征相关
DOI：
10.1186/1475-2867-14-46
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发表时间：
2014
影响因子：
5.8
通讯作者：
Tan WL
中科院分区：
文献类型：
--
作者：
Shi R;Zhao Z;Zhou H;Wei M;Ma WL;Zhou JY;Tan WL
Pin 2/TRF 1结合蛋白X1（PinX 1）是一种内源性端粒酶抑制因子，也是一种主要的单倍不足肿瘤抑制基因。越来越多的证据表明，PinX 1的表达减少在肿瘤发生中起着关键作用。然而，PinX 1的表达状态及其与前列腺癌（PCa）的临床病理特征的相关性尚未研究。采用实时荧光定量RT-PCR（qRT-PCR）和Western blotting检测PCa及癌旁正常前列腺组织中PinX 1 mRNA和蛋白的表达。PinX 1的临床病理意义进行了研究，通过免疫组织化学（IHC）分析PCa组织芯片（TMA）。PinX 1阳性表达的临界评分通过受试者工作特征（ROC）分析确定。采用卡方检验分析PinX 1表达与PCa临床病理特征的相关性。PinX 1 mRNA和蛋白的表达减少，观察在大多数PCa，与他们配对的相邻正常前列腺组织相比。当PinX 1阳性表达率大于60%（ROC曲线下面积= 0.833，P = 0.000）时，正常前列腺组织中PinX 1阳性表达率为100%（8/8），PCa组织中PinX 1阳性表达率为32.5%（13/40）。PinX 1表达降低与临床分期（χ2 = 10.230，p = 0.017）、Gleason评分（χ2 = 4.019，p = 0.045）、区域淋巴结转移（χ2 = 10.852，p = 0.004）和远处转移（χ2 = 7.965，p = 0.005）相关。我们的研究结果表明，PinX 1的表达减少与PCa患者的进展特征相关，并可能作为诊断的潜在标志物。
Pin2/TRF1 binding protein X1 (PinX1) has been identified as an endogenous telomerase inhibitor and a major haploinsufficient tumor suppressor gene. Increasing evidence suggests that reduced expression of PinX1 plays a key role in tumorigenesis. However, the PinX1 expression status and its correlation with the clinicopathological features in prostate cancer (PCa) have not been investigated. PinX1 mRNA and protein expression in PCa and adjacent normal prostate tissues were evaluated by real-time quantitative RT-PCR (qRT-PCR) and western blotting. The clinicopathological significance of PinX1 was investigated by immunohistochemistry (IHC) analysis on a PCa tissue microarray (TMA). The cut-off score for positive expression of PinX1 was determined by the receiver operating characteristic (ROC) analysis. The correlation between PinX1 expression and clinicopathological features of PCa was analyzed by Chi-square test. Reduced expression of PinX1 mRNA and protein was observed in the majority of PCa, compared with their paired adjacent normal prostate tissues. When PinX1 positive expression percentage was determined to be above 60% (area under ROC curve = 0.833, P = 0.000), positive expression of PinX1 was observed in 100% (8/8) of normal prostate tissues and 32.5% (13/40) of PCa tissues by IHC. Reduced expression of PinX1 in patients was correlated with advanced clinical stage (χ2 = 10.230, p = 0.017), high Gleason score (χ2 = 4.019, p = 0.045), positive regional lymph node metastasis (χ2 = 10.852, p = 0.004) and distant metastasis (χ2 = 7.965, p = 0.005). Our findings suggest that reduced expression of PinX1 is correlates to progressive features in patients with PCa and may serve as a potential marker for diagnosis.
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影响因子：
8
作者：
Kondo, T;Oue, N;Yasui, W
通讯作者：
Yasui, W
影响因子：
4.2
作者：
Zuo, Jing;Wang, Da-Hu;Liu, Wei
通讯作者：
Liu, Wei
影响因子：
15.9
作者：
Zhou, Xiao Zhen;Huang, Pengyu;Lu, Kun Ping
通讯作者：
Lu, Kun Ping
通过 mBAFF 靶向递送 PinX1 选择性杀死伯基特淋巴瘤细胞
影响因子：
11.4
作者：
Zhang, L.;Jiang, Y.;Zheng, Y.;Zeng, Y.;Yang, Z.;Huang, G.;Liu, D.;Gao, M.;Shen, X.;Wu, G.;Yan, X.;He, F.
通讯作者：
He, F.
PinX1蛋白表达降低与肿瘤发生发展相关，是卵巢癌新的独立不良预后因素
影响因子：
5.7
作者：
Cai, Mu-Yan;Zhang, Bin;Xie, Dan
通讯作者：
Xie, Dan