Enhanced ROS scavenging and sugar accumulation contribute to drought tolerance of naturally occurring autotetraploids in Poncirus trifoliata
Enhanced ROS scavenging and sugar accumulation contribute to drought tolerance of naturally occurring autotetraploids in Poncirus trifoliata
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增强的ROS清除和糖积累有助于枳实自然发生的同源四倍体的耐旱性
DOI：
10.1111/pbi.13064
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发表时间：
2019-01
影响因子：
13.8
通讯作者：
Liu Ji Hong
中科院分区：
文献类型：
--
作者：
Wei Tonglu;Wang Yue;Xie Zongzhou;Guo Dayong;Chen Chuanwu;Fan Qijun;Deng Xiaodong;Liu Ji Hong
据报道，四倍体表现出更强的抗逆性，但其潜在的分子和生理机制尚不清楚。本研究通过筛选三叶橙（Poncirus trifoliata）的天然幼苗，鉴定出同源四倍体植株。与二倍体相比，四倍体表现出不同的形态和显著的抗旱性和脱水性。转录组分析表明，许多应激反应基因和途径在四倍体中受到不同程度的影响和富集，特别是那些与抗氧化过程和糖代谢相关的酶编码。在干旱条件下，四倍体抗氧化酶（过氧化物酶和超氧化物歧化酶）和蔗糖水解酶（液泡转化酶）的转录物水平和活性升高，同时葡萄糖水平升高，活性氧（ROS）水平降低。这些数据表明，四倍体可能经历了参与ROS清除和糖代谢的基因的广泛转录组重编程，这些基因协同或独立地促进了四倍体的抗逆性增强。我们的研究结果表明，与二倍体相比，四倍体通过提高抗氧化能力和糖积累来维持更强大的活性氧解毒和渗透调节系统，从而比二倍体更具有抗逆性。
Summary Tetraploids have been reported to exhibit increased stress tolerance, but the underlying molecular and physiological mechanisms remain poorly understood. In this study, autotetraploid plants were identified by screening natural seedlings of trifoliate orange (Poncirus trifoliata). The tetraploids exhibited different morphology and displayed significantly enhanced drought and dehydration tolerance in comparison with the diploid progenitor. Transcriptome analysis indicated that a number of stress‐responsive genes and pathways were differentially influenced and enriched in the tetraploids, in particular those coding for enzymes related to antioxidant process and sugar metabolism. Transcript levels and activities of antioxidant enzymes (peroxidase and superoxide dismutase) and sucrose‐hydrolysing enzyme (vacuolar invertase) were increased in the tetraploids upon exposure to the drought, concomitant with greater levels of glucose but lower level of reactive oxygen species (ROS). These data indicate that the tetraploids might undergo extensive transcriptome reprogramming of genes involved in ROS scavenging and sugar metabolism, which contributes, synergistically or independently, to the enhanced stress tolerance of the tetraploid. Our results reveal that the tetraploids take priority over the diploid for stress tolerance by maintaining a more robust system of ROS detoxification and osmotic adjustment via elevating antioxidant capacity and sugar accumulation in comparison with the diploid counterpart.
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影响因子：
5.3
作者：
Tan FQ;Tu H;Liang WJ;Long JM;Wu XM;Zhang HY;Guo WW
通讯作者：
Guo WW
影响因子：
64.5
作者：
Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
6.9
作者：
Jing Wang;Pei Sun;Chun-Li Chen;Yin Wang;Xing-zheng Fu;Ji-Hong Liu
通讯作者：
Jing Wang;Pei Sun;Chun-Li Chen;Yin Wang;Xing-zheng Fu;Ji-Hong Liu
DOI：
10.1177/0032885598078003008
发表时间：
1998-09
期刊：
The Prison Journal
影响因子：
--
作者：
Irina Simonovska;I. Mateescu;V. Manolache
通讯作者：
Irina Simonovska;I. Mateescu;V. Manolache
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen