Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement.
Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement.
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DOI：
10.1186/s12870-015-0450-4
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发表时间：
2015-03-18
影响因子：
5.3
通讯作者：
Guo WW
中科院分区：
文献类型：
--
作者：
Tan FQ;Tu H;Liang WJ;Long JM;Wu XM;Zhang HY;Guo WW
多倍体常被认为赋予植物对环境胁迫的更好适应。四倍体柑橘砧木比二倍体具有更强的抗逆性。大量的柑橘二倍体植株被开发用于柑橘砧木改良。然而，有限的代谢和四倍化相关的分子信息，目前可在系统生物学水平。以资阳香橙（Citrus junos Sieb.）ex Tanaka），是我国特有的柑橘种质资源，广泛用作耐缺铁的柑橘砧木。加倍二倍体紫阳香橙具有典型的形态解剖特征，如株高较矮，叶片大而厚，气孔较大，气孔密度较低。GC-MS（Gas chromatography coupled to mass spectrometry）分析表明，四倍化对叶片中初级代谢产物的积累具有激活作用，其中蔗糖、脯氨酸和γ-氨基丁酸（GABA）等逆境相关代谢产物在加倍二倍体中表达显著上调。然而，LC-QTOF-MS（液相色谱四极杆飞行时间质谱）分析表明，四倍化对叶片中次生代谢产物的积累有抑制作用，所有33个黄酮类化合物在4x中下调，而所有6个黄烷酮类化合物在4x中上调。通过RNA-seq分析，仅发现212个基因（占检测到的基因的0.8%）在2x和4x叶之间显著差异表达。值得注意的是，这些基因与应激反应功能高度相关，包括对盐胁迫、水和脱落酸的反应。有趣的是，转录差异不能解释代谢变化，可能是由于转录后调控。综上所述，四倍化诱导了紫阳香橙叶片初级和次级代谢产物积累的显著变化。然而，四倍化对转录组的影响是有限的。与二倍体相比，加倍二倍体中胁迫相关基因的表达水平和胁迫相关代谢产物的含量更高，这可能有利于加倍二倍体的抗逆性。本文的在线版本（doi：10.1186/s12870-015-0450-4）包含补充材料，可供授权用户使用。
Polyploidy has often been considered to confer plants a better adaptation to environmental stresses. Tetraploid citrus rootstocks are expected to have stronger stress tolerance than diploid. Plenty of doubled diploid citrus plants were exploited from diploid species for citrus rootstock improvement. However, limited metabolic and molecular information related to tetraploidization is currently available at a systemic biological level. This study aimed to evaluate the occurrence and extent of metabolic and transcriptional changes induced by tetraploidization in Ziyang xiangcheng (Citrus junos Sieb. ex Tanaka), which is a special citrus germplasm native to China and widely used as an iron deficiency tolerant citrus rootstock. Doubled diploid Ziyang xiangcheng has typical morphological and anatomical features such as shorter plant height, larger and thicker leaves, bigger stomata and lower stomatal density, compared to its diploid parent. GC-MS (Gas chromatography coupled to mass spectrometry) analysis revealed that tetraploidization has an activation effect on the accumulation of primary metabolites in leaves; many stress-related metabolites such as sucrose, proline and γ-aminobutyric acid (GABA) was remarkably up-regulated in doubled diploid. However, LC-QTOF-MS (Liquid chromatography quadrupole time-of-flight mass spectrometry) analysis demonstrated that tetraploidization has an inhibition effect on the accumulation of secondary metabolites in leaves; all the 33 flavones were down-regulated while all the 6 flavanones were up-regulated in 4x. By RNA-seq analysis, only 212 genes (0.8% of detected genes) are found significantly differentially expressed between 2x and 4x leaves. Notably, those genes were highly related to stress-response functions, including responses to salt stress, water and abscisic acid. Interestingly, the transcriptional divergence could not explain the metabolic changes, probably due to post-transcriptional regulation. Taken together, tetraploidization induced considerable changes in leaf primary and secondary metabolite accumulation in Ziyang xiangcheng. However, the effect of tetraploidization on transcriptome is limited. Compared to diploid, higher expression level of stress related genes and higher content of stress related metabolites in doubled diploid could be beneficial for its stress tolerance. The online version of this article (doi:10.1186/s12870-015-0450-4) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Bombarely A;Edwards KD;Sanchez-Tamburrino J;Mueller LA
通讯作者：
Mueller LA
影响因子：
3.8
作者：
Bassene, J. B.;Froelicher, Y.;Ancillo, G.
通讯作者：
Ancillo, G.
影响因子：
7.7
作者：
Froelicher, Y.;Dambier, D.;Ollitrault, P.
通讯作者：
Ollitrault, P.
影响因子：
3.3
作者：
Auger, DL;Gray, AD;Birchler, JA
通讯作者：
Birchler, JA
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y