HMGB1 level in cerebrospinal fluid as a complimentary biomarker for the diagnosis of tuberculous meningitis.
HMGB1 level in cerebrospinal fluid as a complimentary biomarker for the diagnosis of tuberculous meningitis.
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脑脊液中的 HMGB1 水平作为诊断结核性脑膜炎的补充生物标志物。
DOI：
10.1186/s40064-016-3478-5
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Qin Y
中科院分区：
文献类型：
--
作者：
Chen Y;Zhang J;Wang X;Wu Y;Zhu L;Lu L;Shen Q;Qin Y
高迁移率族蛋白-1（HMGB 1）是一种参与许多病理状态的促炎DAMP蛋白。在这项研究中，我们评估了CSF HMGB 1作为诊断结核性脑膜炎（TBM）的生物标志物的可用性。我们的研究共包括59例TBM患者和169例对照患者。获得CSF样品并使用商业ELISA试剂盒分析HMGB 1。TBM患者（n = 59）的平均CSF HMGB 1为19.36 ng/ml，而非TB脑膜炎患者（n = 30）为3.12 ng/ml，神经外结核患者（n = 73）为2.13 ng/ml，对照组（n = 66）为1.06 ng/ml。根据受试者工作特征曲线计算出3.4ng/ml的临界值，表明CSF HMGB 1单独检测诊断TBM的敏感性和特异性分别为61.02%和89.94%。在神经外结核和TBM高危患者中，CSF HMGB 1似乎是早期鉴别诊断TBM的良好候选者，其临界值为3.8 ng/ml，其敏感性和特异性分别为79.49%和94.52%。我们的发现可能被证明是临床上有用的，因为CSF HMGB 1 ELISA可以在几乎所有的临床实验室进行，特别是当复杂的技术要么耗时或不可用时。
High mobility group box-1 (HMGB1) is a proinflammatory, DAMP protein that participates in many pathological conditions. In this study, we evaluated the usability of CSF HMGB1 as a biomarker for the diagnosis of tuberculous meningitis (TBM). A total of 59 TBM patients and 169 control patients were included in our study. CSF samples were obtained and analyzed for HMGB1 using a commercial ELISA kit. The mean CSF HMGB1 was 19.36 ng/ml in TBM patients (n = 59) versus 3.12 ng/ml in non-TB meningitis patients (n = 30), 2.13 ng/ml in patients with extra neural tuberculosis (n = 73), and 1.06 ng/m in controls (n = 66). According to the receiver operator characteristic curves, a cut-off value of 3.4 ng/ml was calculated, indicating that the sensitivity and specificity of CSF HMGB1 alone in diagnosis of TBM were 61.02 and 89.94 %, respectively. In patients with extra neural tuberculosis and a high risk of TBM, CSF HMGB1 seemed to be a good candidate for early differential diagnosis of TBM at the cut-off value of 3.8 ng/ml, when the sensitivity and specificity were 79.49 and 94.52 % respectively. Our finding may prove to be clinically useful, because CSF HMGB1 ELISA can be performed in almost all clinical laboratories, especially when sophisticated technologies are either time consuming or unavailable.
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HMGB1-LPS 复合物促进骨关节炎滑膜成纤维细胞向类风湿关节炎滑膜成纤维细胞样表型的转化。
影响因子：
9
作者：
通讯作者：
--
DOI：
10.5588/ijtld.15.0253
发表时间：
2015-10
期刊：
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
影响因子：
--
作者：
Bahr NC;Tugume L;Rajasingham R;Kiggundu R;Williams DA;Morawski B;Alland D;Meya DB;Rhein J;Boulware DR
通讯作者：
Boulware DR
影响因子：
64.8
作者：
Scaffidi, P;Misteli, T;Bianchi, ME
通讯作者：
Bianchi, ME
影响因子：
7.4
作者：
Chung HW;Lee SG;Kim H;Hong DJ;Chung JB;Stroncek D;Lim JB
通讯作者：
Lim JB
影响因子：
4.9
作者：
Palone, Francesca;Vitali, Roberta;Stronati, Laura
通讯作者：
Stronati, Laura