Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns.
Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns.
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棉花蔗糖合酶基因家族分析：结构、系统发育和表达模式
DOI：
10.1186/1471-2229-12-85
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发表时间：
2012-06-13
影响因子：
5.3
通讯作者：
Rong J
中科院分区：
文献类型：
--
作者：
Chen A;He S;Li F;Li Z;Ding M;Liu Q;Rong J
研究背景蔗糖合成酶（Sucrose Synthase，Sus）是植物蔗糖代谢的关键酶。几个旁系同源基因编码不同的同工酶的Sus已被确定，并在多个植物基因组的特点，而有限的信息，Sus基因是迄今为cotton.ResultsHere，我们报告的分子克隆，结构组织，系统进化和表达谱的7个Sus基因（GaSus1至7）确定从二倍体纤维棉（亚洲棉）。cDNA和基因组序列的比较表明，棉花GaSusgenes被多个内含子中断。同源基因内含子的比较筛选表明，在棉花和其他更远的植物物种中的Sus基因的内含子的数量和位置是高度保守的。系统发育分析表明，GaSus1、GaSus2、GaSus3、GaSus4和GaSus5可聚为双子叶植物类群，GaSus6和GaSus7则均匀地分为双子叶植物和单子叶植物类群。对7个Sus基因的表达谱分析表明，除GaSus2在所有组织中均检测不到转录本，GaSus7仅在茎和花瓣中表达外，其余5个旁系同源基因在棉纤维细胞中均表现出发育依赖性的表达谱。本研究结果为棉花Sus基因家族在纤维生长发育过程中的进化保守性和亚功能分化提供了新的认识。
BackgroundIn plants, sucrose synthase (Sus) is widely considered as a key enzyme involved in sucrose metabolism. Several paralogous genes encoding different isozymes of Sus have been identified and characterized in multiple plant genomes, while limited information of Sus genes is available to date for cotton.ResultsHere, we report the molecular cloning, structural organization, phylogenetic evolution and expression profiles of seven Sus genes (GaSus1to 7) identified from diploid fiber cotton (Gossypium arboreum). Comparisons between cDNA and genomic sequences revealed that the cottonGaSusgenes were interrupted by multiple introns. Comparative screening of introns in homologous genes demonstrated that the number and position of Sus introns are highly conserved among Sus genes in cotton and other more distantly related plant species. Phylogenetic analysis showed thatGaSus1,GaSus2,GaSus3,GaSus4andGaSus5could be clustered together into a dicot Sus group, whileGaSus6andGaSus7were separated evenly into other two groups, with members from both dicot and monocot species. Expression profiles analyses of the seven Sus genes indicated that exceptGaSus2, of which the transcripts was undetectable in all tissues examined, andGaSus7, which was only expressed in stem and petal, the other five paralogues were differentially expressed in a wide ranges of tissues, and showed development-dependent expression profiles in cotton fiber cells.ConclusionsThis is a comprehensive study of the Sus gene family in cotton plant. The results presented in this work provide new insights into the evolutionary conservation and sub-functional divergence of the cotton Sus gene family in response to cotton fiber growth and development.
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DOI：
10.1073/pnas.0900188106
发表时间：
2009-08-04
影响因子：
11.1
作者：
Coleman, Heather D.;Yan, Jimmy;Mansfield, Shawn D.
通讯作者：
Mansfield, Shawn D.
影响因子：
4.2
作者：
Harada, T;Satoh, S;Ishizawa, K
通讯作者：
Ishizawa, K
影响因子：
5.1
作者：
Carlson, SJ;Chourey, PS;Datta, R
通讯作者：
Datta, R
影响因子：
5.2
作者：
Hirose, Tatsuro;Scofield, Graham N.;Terao, Tomio
通讯作者：
Terao, Tomio
影响因子：
6.5
作者：
Hardin, SC;Huber, SC
通讯作者：
Huber, SC