Endothelial Cell Membrane Sensitivity to Shear Stress is Lipid Domain Dependent.
Endothelial Cell Membrane Sensitivity to Shear Stress is Lipid Domain Dependent.
复制标题
DOI：
10.1007/s12195-010-0136-9
复制
发表时间：
2011-06-01
影响因子：
2.8
通讯作者：
Butler, Peter J.
中科院分区：
文献类型：
--
作者：
Tabouillot, Tristan;Muddana, Hari S.;Butler, Peter J.
关键词：
血流相关的剪切应力导致内皮细胞的生理和病理生理生化过程，这些过程可能是由质膜脂质结构域的改变引发的，其特征在于液体有序（lo），如筏或小窝，或液体无序（ld）。为了测试域依赖性剪切敏感性，我们使用时间相关单光子计数仪器来评估域选择性脂质类似物DiI-C12和DiI-C18在受到生理流体剪切应力的内皮细胞中的物理学和动力学。在静态条件下，DiI-C12的荧光寿命小于DiI-C18的荧光寿命，DiI-C12的扩散系数大于DiI-C18的扩散系数，证实DiI-C12探针1d，更流动的膜环境，DiI-C18探针1 o相。由DiI-C12探测的结构域在剪切开始后表现出荧光寿命的早期（10 s）和瞬时降低，而由DiI-C18探测的结构域表现出荧光寿命的延迟降低，其持续细胞经受流动的2 min。剪切作用后，DiI-C18的扩散系数增加，而DiI-C12的扩散系数保持不变。在控制体积中的分子数（N）的测定表明，DiI-C12标记的结构域增加后，立即在N阶跃剪切，而N的DiI-C18染色的膜短暂下降。这些结果表明，膜微区对流体剪切应力的敏感性不同。
Blood flow-associated shear stress causes physiological and pathophysiological biochemical processes in endothelial cells that may be initiated by alterations in plasma membrane lipid domains characterized as liquid-ordered (lo), such as rafts or caveolae, or liquid-disordered (ld). To test for domain–dependent shear sensitivity, we used time-correlated single photon counting instrumentation to assess the photophysics and dynamics of the domain-selective lipid analogues DiI-C12 and DiI-C18 in endothelial cells subjected to physiological fluid shear stress. Under static conditions, DiI-C12 fluorescence lifetime was less than DiI-C18 lifetime and the diffusion coefficient of DiI-C12 was greater than the DiI-C18 diffusion coefficient, confirming that DiI-C12 probes ld, a more fluid membrane environment, and DiI-C18 probes the lo phase. Domains probed by DiI-C12 exhibited an early (10 s) and transient decrease of fluorescence lifetime after the onset of shear while domains probed by DiI-C18 exhibited a delayed decrease of fluorescence lifetime that was sustained for the 2 min the cells were subjected to flow. The diffusion coefficient of DiI-C18 increased after shear imposition, while that of DiI-C12 remained constant. Determination of the number of molecules (N) in the control volume suggested that DiI-C12-labeled domains increased in N immediately after step-shear, while N for DiI-C18-stained membrane transiently decreased. These results demonstrate that membrane microdomains are differentially sensitive to fluid shear stress.
登录
查看更多内容
DOI：
10.1152/ajpheart.2000.278.5.h1598
发表时间：
2000-05-01
影响因子：
4.8
作者：
Bao, XP;Clark, CB;Frangos, JA
通讯作者：
Frangos, JA
影响因子：
2
作者：
Duncan RR;Bergmann A;Cousin MA;Apps DK;Shipston MJ
通讯作者：
Shipston MJ
DOI：
10.1161/01.atv.12.11.1254
发表时间：
1992-11-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
DEPAOLA, N;GIMBRONE, MA;DEWEY, CF
通讯作者：
DEWEY, CF
影响因子：
5.5
作者：
Dangaria, Jhanvi H.;Butler, Peter J.
通讯作者：
Butler, Peter J.
DOI：
10.1006/bbrc.1996.1081
发表时间：
1996-07-25
影响因子：
3.1
作者：
Frangos, JA;Huang, TY;Clark, CB
通讯作者：
Clark, CB