Biomineralization of a self-assembled extracellular matrix for bone tissue engineering.
Biomineralization of a self-assembled extracellular matrix for bone tissue engineering.
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DOI：
10.1089/ten.tea.2007.0371
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发表时间：
2009-02
影响因子：
--
通讯作者：
Pernodet N
中科院分区：
文献类型：
--
作者：
Meng Y;Qin YX;DiMasi E;Ba X;Rafailovich M;Pernodet N
Understanding how biomineralization occurs in the extracellular matrix (ECM) of bone cells is crucial to the understanding of bone formation and the development of a successfully engineered bone tissue scaffold. It is still unclear how ECM mechanical properties affect protein–mineral interactions in early stages of bone mineralization. We investigated the longitudinal mineralization properties of MC3T3-E1 cells and the elastic modulus of their ECM using shear modulation force microscopy, synchrotron grazing incidence X-ray diffraction (GIXD), scanning electron microscopy, energy dispersive X-ray spectroscopy, and confocal laser scanning microscopy (CLSM). The elastic modulus of the ECM fibers underwent significant changes for the mineralizing cells, which were not observed in the nonmineralizing cells. On substrates conducive to ECM network production, the elastic modulus of mineralizing cells increased at time points corresponding to mineral production, whereas that of the nonmineralizing cells did not vary over time. The presence of hydroxyapatite in mineralizing cells and the absence thereof in the nonmineralizing ones were confirmed by GIXD, and CLSM showed that a restructuring of actin occurred only for mineral-producing cells. These results show that the correct and complete development of the ECM network is required for osteoblasts to mineralize. This in turn requires a suitably prepared synthetic substrate for bone development to succeed in vitro.
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影响因子：
14
作者：
Declerq, HA;Verbeeck, RMH;Cornelissen, MJ
通讯作者：
Cornelissen, MJ
影响因子：
6.2
作者：
Kuhn, LT;Xu, YT;Glimcher, MJ
通讯作者：
Glimcher, MJ
磷酸钙纳米颗粒结晶度对骨髓间充质干细胞粘附、增殖和分化的影响
影响因子：
--
作者：
Hu, Qinghong;Tan, Zhou;Tang, Ruikang
通讯作者：
Tang, Ruikang
影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
影响因子：
4.8
作者：
García, AJ;Takagi, J;Boettiger, D
通讯作者：
Boettiger, D