Effect of puromycin aminonucleoside on HSPG core protein content of glomerular epithelial cells.
Effect of puromycin aminonucleoside on HSPG core protein content of glomerular epithelial cells.
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嘌呤霉素氨基核苷对肾小球上皮细胞HSPG核心蛋白含量的影响
DOI：
10.1152/ajprenal.1988.255.4.f590
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Lewis,EJ
中科院分区：
文献类型：
--
作者：
Kasinath,BS;Singh,AK;Kanwar,YS;Lewis,EJ
It has been suggested that the glomerular basement membrane heparan sulfate proteoglycan (HSPG) is an important determinant of the glomerular permselectivity barrier. Derangements in the content of basement membrane heparan sulfate have been implicated in alterations in glomerular permselectivity seen in many glomerular diseases such as aminonucleoside nephrosis. The cellular origin and metabolism of the glomerular basement membrane HSPG have not been studied in detail. We have detected the expression of the proteoglycan by cloned glomerular visceral epithelial cells of the rat by employing a specific antibody against the core protein of HSPG isolated from the rat glomerular basement membrane. These findings suggest that in the rat in vivo glomerular visceral epithelial cells are one source of heparan sulfate present in the glomerular basement membrane. The effect of puromycin aminonucleoside (PAN) on the HSPG core protein content of the cloned glomerular epithelial cells was studied. By a quantitative immunoperoxidase method, the aminonucleoside caused a 28% reduction in the core protein content of the epithelial cells (P less than 0.01) following 72 h of incubation. However, the content of Heymann nephritis-related antigen, Fx1A was unchanged. Studies employing [3H]leucine incorporation showed that PAN was a weak inhibitor of de novo protein synthesis at 24 h of incubation, with complete recovery at 48 and 72 h. These data suggest that PAN effect on heparan sulfate core protein cannot be attributed to generalized inhibition of protein synthesis. The precise mechanism underlying the aminonucleoside effect on heparan sulfate core protein remains to be elucidated.
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由溶组织内阿米巴引起的肠道疾病。
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
J. Ravdin
通讯作者：
J. Ravdin
溶组织内阿米巴半乳糖和 N-乙酰基-D-半乳糖胺抑制粘附凝集素的亚基结构。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
PetriJr,WA;Chapman,MD;Snodgrass,T;Mann,BJ;Broman,J;Ravdin,JI
通讯作者：
Ravdin,JI
从溶组织内阿米巴毒株中分离出具有凝集素特性的 220 千道尔顿蛋白质。
影响因子：
6.4
作者：
J. L. Rosales;I. Meza;A. López;P. Talamás;M. Rojkind
通讯作者：
M. Rojkind
非致病性溶组织内阿米巴克隆培养物在轴化过程中同工酶模式的变化
影响因子：
3.1
作者：
D. Mirelman;R. Bracha;A. Wexler;A. Chayen
通讯作者：
A. Chayen
人类阿米巴病的病理学。
影响因子：
3.3
作者：
H. Brandt;R. P. Tamayo
通讯作者：
R. P. Tamayo