In Vitro Maturation and In Vivo Integration and Function of an Engineered Cell-Seeded Disc-like Angle Ply Structure (DAPS) for Total Disc Arthroplasty.
In Vitro Maturation and In Vivo Integration and Function of an Engineered Cell-Seeded Disc-like Angle Ply Structure (DAPS) for Total Disc Arthroplasty.
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DOI：
10.1038/s41598-017-15887-4
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发表时间：
2017-11-17
影响因子：
4.6
通讯作者：
Mauck RL
中科院分区：
文献类型：
--
作者：
Martin JT;Gullbrand SE;Kim DH;Ikuta K;Pfeifer CG;Ashinsky BG;Smith LJ;Elliott DM;Smith HE;Mauck RL
全椎间盘置换术是治疗晚期椎间盘疾病的一种很有前途的方法。为了实现这一目标，我们开发了细胞种子盘状角层结构（DAPS），并通过体外研究表明，这些结构成熟，与长期培养的天然盘的组成、结构和功能相匹配。然后，我们在全椎间盘置换术大鼠体内模型中评估了DAPS的性能；在体内5周后，DAPS保持其结构，防止椎间骨融合，并在原位生理负荷下匹配天然椎间盘的力学功能。然而，DAPS在植入后迅速失去了蛋白多糖，并且没有融入邻近的椎骨。为了解决这个问题，我们修改了设计，加入了聚合物终板，将DAPS与邻近的椎骨连接起来，并表明这种修改减轻了体内蛋白聚糖的损失，同时保持了机械功能并促进了整合。总之，这些数据表明细胞种子工程椎间盘可以复制天然椎间盘的许多特征，是全椎间盘置换术的可行选择。
Total disc replacement with an engineered substitute is a promising avenue for treating advanced intervertebral disc disease. Toward this goal, we developed cell-seeded disc-like angle ply structures (DAPS) and showed through in vitro studies that these constructs mature to match native disc composition, structure, and function with long-term culture. We then evaluated DAPS performance in an in vivo rat model of total disc replacement; over 5 weeks in vivo, DAPS maintained their structure, prevented intervertebral bony fusion, and matched native disc mechanical function at physiologic loads in situ. However, DAPS rapidly lost proteoglycan post-implantation and did not integrate into adjacent vertebrae. To address this, we modified the design to include polymer endplates to interface the DAPS with adjacent vertebrae, and showed that this modification mitigated in vivo proteoglycan loss while maintaining mechanical function and promoting integration. Together, these data demonstrate that cell-seeded engineered discs can replicate many characteristics of the native disc and are a viable option for total disc arthroplasty.
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影响因子：
9.7
作者：
Kim, Dong Hwa;Martin, John T.;Elliott, Dawn M.;Smith, Lachlan J.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.
影响因子：
14
作者：
Bian, Liming;Zhai, David Y.;Tous, Elena;Rai, Reena;Mauck, Robert L.;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
7
作者：
Bian L;Angione SL;Ng KW;Lima EG;Williams DY;Mao DQ;Ateshian GA;Hung CT
通讯作者：
Hung CT
DOI：
10.1073/pnas.1214100110
发表时间：
2013-06-18
影响因子：
11.1
作者：
Bian, Liming;Guvendiren, Murat;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
5.6
作者：
Dickhut, Andrea;Pelttari, Karoliina;Richter, Wiltrud
通讯作者：
Richter, Wiltrud