Phenotypic stability, matrix elaboration and functional maturation of nucleus pulposus cells encapsulated in photocrosslinkable hyaluronic acid hydrogels.
Phenotypic stability, matrix elaboration and functional maturation of nucleus pulposus cells encapsulated in photocrosslinkable hyaluronic acid hydrogels.
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DOI：
10.1016/j.actbio.2014.10.030
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发表时间：
2015-01
影响因子：
9.7
通讯作者：
Mauck, Robert L.
中科院分区：
文献类型：
--
作者：
Kim, Dong Hwa;Martin, John T.;Elliott, Dawn M.;Smith, Lachlan J.;Mauck, Robert L.
关键词：
髓核（NP）的退化是椎间盘退变的早期标志。由于无血管组织的细胞结构低且营养供应不良，NP 的内源再生能力受到限制。为了恢复 NP，人们已经探索了许多用于细胞递送的生物材料。这些材料必须支持 NP 细胞表型，同时促进在尽可能短的时间内制作出类似 NP 的细胞外基质。我们之前对软骨细胞和间充质干细胞的研究表明，基于透明质酸（HA）的水凝胶可有效促进基质产生和功能材料特性的发展。然而，这种材料尚未在 NP 细胞的背景下进行评估。因此，为了测试该材料的 NP 再生能力，将牛 NP 细胞以低接种密度（20 × 106 个细胞/ml）或高接种密度（60 × 106 个细胞/ml）封装在 1% w/vol HA 水凝胶中，并将构建体培养 8 周。这些工程化的负载 NP 细胞的 HA 水凝胶显示出功能性基质积累，在两种接种密度下，随着培养时间的推移，基质含量和机械性能不断增加。此外，封装的细胞显示出 NP 特异性基因表达谱，该表达谱显着高于封装前扩增的 NP 细胞，表明表型的恢复。有趣的是，与较高播种密度相比，较低播种密度时这些水平较高。这些发现支持使用基于 HA 的水凝胶进行 NP 组织工程和细胞疗法，旨在恢复或替换内源性 NP。
Degradation of the nucleus pulposus (NP) is an early hallmark of intervertebral disc degeneration. The capacity for endogenous regeneration in the NP is limited due to the low cellularity and poor nutrient supply of this avascular tissue. Towards restoring the NP, a number of biomaterials have been explored for cell delivery. These materials must support the NP cell phenotype while promoting the elaboration of an NP-like extracellular matrix in the shortest possible time. Our previous work with chondrocytes and mesenchymal stem cells demonstrated that hydrogels based on hyaluronic acid (HA) are effective at promoting matrix production and the development of functional material properties. However, this material has not been evaluated in the context of NP cells. Therefore, to test this material for NP regeneration, bovine NP cells were encapsulated in 1% w/vol HA hydrogels at either a low seeding density (20 × 106 cells/ml) or a high seeding density (60 × 106 cells/ml), and constructs were cultured over an 8 week period. These engineered NP cell-laden HA hydrogels showed functional matrix accumulation, with increasing matrix content and mechanical properties with time in culture at both seeding densities. Furthermore, encapsulated cells showed NP-specific gene expression profiles that were significantly higher than expanded NP cells prior to encapsulation, suggesting a restoration of phenotype. Interestingly, these levels were higher at the lower seeding density compared to the higher seeding density. These findings support the use of HA-based hydrogels for NP tissue engineering and cellular therapies directed at restoration or replacement of the endogenous NP.
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影响因子：
2.4
作者：
Huang AH;Farrell MJ;Mauck RL
通讯作者：
Mauck RL
影响因子：
5.3
作者：
Lefebvre, V;Huang, WD;deCrombrugghe, B
通讯作者：
deCrombrugghe, B
DOI：
10.1073/pnas.1214100110
发表时间：
2013-06-18
影响因子：
11.1
作者：
Bian, Liming;Guvendiren, Murat;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
14
作者：
Kim, Iris L.;Mauck, Robert L.;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
3
作者：
Kluba, T;Niemeyer, T;Gründer, T
通讯作者：
Gründer, T