Molecular Characterization of Rice OsLCB2a1 Gene and Functional Analysis of its Role in Insect Resistance.
Molecular Characterization of Rice OsLCB2a1 Gene and Functional Analysis of its Role in Insect Resistance.
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水稻OsLCB2a1基因的分子特征及其抗虫作用的功能分析
DOI：
10.3389/fpls.2016.01789
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zhu ZR
中科院分区：
文献类型：
--
作者：
Begum MA;Shi XX;Tan Y;Zhou WW;Hannun Y;Obeid L;Mao C;Zhu ZR
在植物中，鞘脂类，如长链碱基（LCBs），在逆境反应中起生物活性分子的作用。到目前为止，尚不清楚这些脂质是否与生物对草食动物的应激反应有关。本文报道了水稻LCB基因OsLCB2a1编码丝氨酸棕榈酰转移酶（SPT）亚基，SPT是负责鞘脂新生生物合成的关键酶，在植物对棕色飞虱（BPH）攻击的防御反应中起关键作用，其上调可保护植物免受食草动物的侵害。水稻幼苗中OsLCB2a1基因的转录量在BPH发作后4 h增加，但在8-24 h下降。鞘脂测量分析显示，与野生型（WT）植物相比，OsLCB2a1在拟南芥中的过表达使三羟基化LCB植物鞘磷脂（t18:0）和植物神经酰胺分别增加1.7倍和1.3倍。转基因拟南芥植株叶片的胼胝体和蜡沉积量也高于WT植株。在拟南芥中过表达OsLCB2a1基因可降低青桃蚜（Myzus persicae）种群规模。此外，电渗透图（EPG）结果表明，蚜虫在到达转基因植株的韧皮部时遇到了抗性因素。在oslcb2a1过表达的转基因植株中，与水杨酸信号通路相关的防御反应基因保持上调。我们的数据强调了OsLCB2a1在植物生物应激反应中的关键功能。
In plants, sphingolipids, such as long-chain bases (LCBs), act as bioactive molecules in stress responses. Until now, it is still not clear if these lipids are involved in biotic stress responses to herbivore. Herein we report that a rice LCB gene, OsLCB2a1 encoding a subunit of serine palmitoyltransferase (SPT), a key enzyme responsible for the de novo biosynthesis of sphingolipids, plays a critical role in plant defense response to the brown planthopper (BPH) attack and that its up-regulation protects plants from herbivore infestation. Transcripts of OsLCB2a1 gene in rice seedlings were increased at 4 h, but decreased at 8–24 h after BPH attack. Sphingolipid measurement profiling revealed that overexpression of OsLCB2a1 in Arabidopsis thaliana increased trihydroxylated LCB phytosphingosine (t18:0) and phytoceramide by 1.7 and 1.3-fold, respectively, compared with that of wild type (WT) plants. Transgenic Arabidopsis plants also showed higher callose and wax deposition in leaves than that of WT. Overexpression of OsLCB2a1 gene in A. thaliana reduced the population size of green peach aphid (Myzus persicae). Moreover, the electrical penetration graph (EPG) results indicated that the aphids encounter resistance factors while reaching for the phloem on the transgenic plants. The defense response genes related to salicylic acid signaling pathway, remained uplgulated in the OsLCB2a1-overexpressing transgenic plants. Our data highlight the key functions of OsLCB2a1 in biotic stress response in plants.
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影响因子：
7.4
作者：
Hao, Peiying;Liu, Caixiang;He, Guangcun
通讯作者：
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DOI：
10.1016/j.bbrc.2011.06.028
发表时间：
2011-07-08
影响因子：
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作者：
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