Identification of cucurbit chlorotic yellows virus P4.9 as a possible movement protein
Identification of cucurbit chlorotic yellows virus P4.9 as a possible movement protein
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鉴定葫芦褪绿黄化病毒 P4.9 作为可能的运动蛋白
DOI:
10.1186/s12985-019-1192-y
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发表时间:
2019-06
期刊:
影响因子:
4.8
通讯作者:
Shi Yan
中科院分区:
文献类型:
--
作者:
Wei Ying;Shi Yajuan;Han Xaioyu;Chen Siyu;Li Honglian;Chen Linlin;Sun Bingjian;Shi Yan
Background:Cucurbit chlorotic yellows virus (CCYV) is a bipartite cucurbit-infecting crinivirus within the family Closteroviridae. The crinivirus genome varies among genera. P4.9 is the first protein encoded by CCYV RNA2. P5, which is encoded by LIYV, is necessary for efficient viral infectivity in plants; however, it remains unknown whether CCYV P4.9 is involved in movement.Finding:In this study, we used green fluorescent protein (GFP) to examine the intracellular distribution of P4.9-GFP in plant cells, and observed fluorescence in the cytoplasm and nucleus. Transient expression of P4.9 was localized to the plasmodesmata. Co-infiltration of agrobacterium carrying binary plasmids of P4.9 and GFP facilitated GFP diffusion between cells. Besides P4.9 was able to spread by itself to neighboring cells, and co-localized with a marker specific to the endoplasmic reticulum, HDEL-mCherry, but not with the Golgi marker Man49-mCherry.Conclusions:Together, these results demonstrate that CCYV P4.9 is involved in cell-cell movement.
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影响因子:
3.7
作者:
S. Tatineni;C. Robertson;S. Garnsey;M. Bar-joseph;S. Gowda;W. Dawson
通讯作者:
S. Tatineni;C. Robertson;S. Garnsey;M. Bar-joseph;S. Gowda;W. Dawson
DOI:
10.1073/pnas.96.26.14771
发表时间:
1999-12-21
影响因子:
11.1
作者:
Peremyslov, VV;Hagiwara, Y;Dolja, VV
通讯作者:
Dolja, VV
影响因子:
5.4
作者:
Peremyslov, VV;Pan, YW;Dolja, VV
通讯作者:
Dolja, VV
影响因子:
6.4
作者:
Qiao W;Medina V;Kuo YW;Falk BW
通讯作者:
Falk BW
影响因子:
3.5
作者:
Guenoune-Gelbart, Dana;Elbaum, Michael;Epel, Bernard L.
通讯作者:
Epel, Bernard L.