Identification of cucurbit chlorotic yellows virus P4.9 as a possible movement protein

Identification of cucurbit chlorotic yellows virus P4.9 as a possible movement protein
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鉴定葫芦褪绿黄化病毒 P4.9 作为可能的运动蛋白

DOI:
10.1186/s12985-019-1192-y
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发表时间:
2019-06
期刊:
影响因子:
4.8
通讯作者:
Shi Yan
Shi Yan
中科院分区:
医学3区
文献类型:
--
作者:
Wei Ying;Shi Yajuan;Han Xaioyu;Chen Siyu;Li Honglian;Chen Linlin;Sun Bingjian;Shi Yan

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背景:瓜类褪绿黄化病毒(Cucurbit chlorotic yellows virus,CCYV)是一种由梭状病毒科(Closteroviridae)的一种二分型侵染葫芦科植物的病毒。海百合病毒的基因组在不同的属中有所不同。P4.9是CCYV RNA 2编码的第一个蛋白。P5是由LIYV编码的,是病毒在植物中有效感染所必需的,然而CCYV P4.9是否参与了植物的运动还不清楚。P4.9的瞬时表达定位于胞间连丝。携带P4.9和GFP双质粒的农杆菌的共浸润促进GFP在细胞之间的扩散。此外,P4.9能够通过自身传播到邻近细胞,并与内质网特异性标记物HDEL-mCherry共定位,但不与高尔基体标记物Man 49-mCherry.Conclusions:总之,这些结果表明,CCYV P4.9参与细胞-细胞运动。
Background:Cucurbit chlorotic yellows virus (CCYV) is a bipartite cucurbit-infecting crinivirus within the family Closteroviridae. The crinivirus genome varies among genera. P4.9 is the first protein encoded by CCYV RNA2. P5, which is encoded by LIYV, is necessary for efficient viral infectivity in plants; however, it remains unknown whether CCYV P4.9 is involved in movement.Finding:In this study, we used green fluorescent protein (GFP) to examine the intracellular distribution of P4.9-GFP in plant cells, and observed fluorescence in the cytoplasm and nucleus. Transient expression of P4.9 was localized to the plasmodesmata. Co-infiltration of agrobacterium carrying binary plasmids of P4.9 and GFP facilitated GFP diffusion between cells. Besides P4.9 was able to spread by itself to neighboring cells, and co-localized with a marker specific to the endoplasmic reticulum, HDEL-mCherry, but not with the Golgi marker Man49-mCherry.Conclusions:Together, these results demonstrate that CCYV P4.9 is involved in cell-cell movement.
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