Antitumor effect of Kanglaite® injection in human pancreatic cancer xenografts.
Antitumor effect of Kanglaite® injection in human pancreatic cancer xenografts.
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DOI：
10.1186/1472-6882-14-228
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发表时间：
2014-07-08
影响因子：
--
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Liu Y;Zhang W;Wang XJ;Liu S
康莱特®注射液（KLT）以薏苡仁油（一种传统中药）为主要成分，已在中国广泛用于癌症治疗。KLT对多种肿瘤具有抑制作用，并且PI 3 K/Akt/mTOR信号促进癌细胞的细胞存活、增殖和进展。因此，靶向这一途径可能会导致开发新的人类癌症治疗方法。在这里，我们研究了KLT对小鼠胰腺癌异种移植物中PI 3 K/Akt/mTOR通路的影响，并评估了其治疗潜力。检测移植瘤的生长和凋亡情况，RT-PCR和Western blotting分别检测PI 3 K/Akt/mTOR通路相关基因和蛋白的表达水平。我们的结果表明，KLT显着抑制胰腺癌异种移植物的生长，同时诱导细胞凋亡。此外，它下调磷酸化Akt和磷酸化mTOR的表达。这些结果表明KLT可以抑制胰腺癌异种移植瘤的生长并诱导其凋亡。KLT还可下调磷酸化Akt和磷酸化mTOR的表达，从而调节PI 3 K/Akt/mTOR信号通路。
Kanglaite® injection (KLT), with a main ingredient of Coix seed oil (a traditional Chinese medicine), has been widely used for cancer treatment in China. KLT has an inhibitory effect on many kinds of tumors and PI3K/Akt/mTOR signaling promotes cell survival, proliferation, and progression in cancer cells. Therefore, targeting this pathway may lead to the development of novel therapeutic approaches for human cancers. Here, we examined the effects of KLT on the PI3K/Akt/mTOR pathway in pancreatic cancer xenografts in mice, and assessed its therapeutic potential. Growth and apoptosis of tumor xenografts were examined, and the expression levels of genes and proteins involved in the PI3K/Akt/mTOR pathway were measured by RT-PCR and western blotting, respectively. Our results revealed that KLT dramatically inhibited the growth of pancreatic cancer xenografts and induced apoptosis simultaneously. Furthermore, it downregulated the expression of phospho-Akt and phospho-mTOR. These results suggest that KLT can suppress growth and induce apoptosis of pancreatic cancer xenografts. Moreover, KLT can downregulate the expression of phospho-Akt and phospho-mTOR to modulate the PI3K/Akt/mTOR signaling pathway.
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DOI：
10.1186/1756-9966-27-31
发表时间：
2008-08-21
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Lu Y;Li CS;Dong Q
通讯作者：
Dong Q
影响因子：
5.4
作者：
Pan, Pei;Wu, Yan;Yuan, Yong-Fang
通讯作者：
Yuan, Yong-Fang
影响因子：
5.7
作者：
Guo, H. Y.;Cai, Y.;Hu, X. C.
通讯作者：
Hu, X. C.
影响因子：
4.8
作者：
Karar J;Maity A
通讯作者：
Maity A
影响因子：
--
作者：
Zhan, Yi-Ping;Huang, Xin-En;Ye, Li-Hong
通讯作者：
Ye, Li-Hong