Transcriptome display during tilapia sex determination and differentiation as revealed by RNA-Seq analysis.
Transcriptome display during tilapia sex determination and differentiation as revealed by RNA-Seq analysis.
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RNA-Seq 分析揭示罗非鱼性别决定和分化过程中的转录组显示
DOI：
10.1186/s12864-018-4756-0
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发表时间：
2018-05-15
期刊：
影响因子：
4.4
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Tao W;Chen J;Tan D;Yang J;Sun L;Wei J;Conte MA;Kocher TD;Wang D
决定硬骨鱼性别的因素多种多样。人们已经做出了巨大的努力来描述不同物种的潜在遗传网络。然而，在硬骨鱼中只发现了7种主要的性别决定基因。虽然已经研究了一些参与性别决定和分化的基因的功能，但我们还远远没有完全了解基因在这一过程中如何相互作用以协调。为了系统地了解鱼类的性别分化，我们从罗非鱼在5、20、30、40、90和180天（孵化后几天）以及45和90天（处理后几天）的性腺转录组中生成了一个动态共表达网络，并将基因表达谱与发育和性别分化联系起来。雌雄生殖腺在5日龄和20日龄时的转录组学特征除了少数参与性别决定的基因外，具有高度的相似性，而在90日龄至180日龄时，雌雄生殖腺的转录组学特征发生了剧烈变化，有一组参与性腺分化和配子体发生的基因表达不同。加权基因相关网络分析发现，borealin、Gtsf1、tesk1、Zar1、Cdn15和rplin的表达变化与foxl2、Cyp19a1a、Gsdf、Dmrt1和amh等先前已知参与性别分化的基因的表达相关。结论罗非鱼性腺基因在性别决定和分化过程中的表达动力学已被广泛研究。这些发现对硬骨鱼性别决定和性别分化的遗传框架提供了深入的见解，并扩展了我们目前对硬骨鱼性别决定过程中发育途径的理解。
BackgroundThe factors determining sex in teleosts are diverse. Great efforts have been made to characterize the underlying genetic network in various species. However, only seven master sex-determining genes have been identified in teleosts. While the function of a few genes involved in sex determination and differentiation has been studied, we are far from fully understanding how genes interact to coordinate in this process.ResultsTo enable systematic insights into fish sexual differentiation, we generated a dynamic co-expression network from tilapia gonadal transcriptomes at 5, 20, 30, 40, 90, and 180 dah (days after hatching), plus 45 and 90 dat (days after treatment) and linked gene expression profiles to both development and sexual differentiation. Transcriptomic profiles of female and male gonads at 5 and 20 dah exhibited high similarities except for a small number of genes that were involved in sex determination, while drastic changes were observed from 90 to 180 dah, with a group of differently expressed genes which were involved in gonadal differentiation and gametogenesis. Weighted gene correlation network analysis identified changes in the expression ofBorealin, Gtsf1, tesk1, Zar1,Cdn15, andRplthat were correlated with the expression of genes previously known to be involved in sex differentiation, such asFoxl2,Cyp19a1a,Gsdf,Dmrt1, andAmh.ConclusionsGlobal gonadal gene expression kinetics during sex determination and differentiation have been extensively profiled in tilapia. These findings provide insights into the genetic framework underlying sex determination and sexual differentiation, and expand our current understanding of developmental pathways during teleost sex determination.
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WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
4.5
作者：
Lavery R;Chassot AA;Pauper E;Gregoire EP;Klopfenstein M;de Rooij DG;Mark M;Schedl A;Ghyselinck NB;Chaboissier MC
通讯作者：
Chaboissier MC
DOI：
10.1016/j.jsbmb.2011.12.015
发表时间：
2012-05-01
影响因子：
4.1
作者：
Glait-Santar, C.;Benayahu, D.
通讯作者：
Benayahu, D.
影响因子：
4
作者：
Barske, Lindsey A.;Capel, Blanche
通讯作者：
Capel, Blanche
影响因子：
4.4
作者：
Conte MA;Gammerdinger WJ;Bartie KL;Penman DJ;Kocher TD
通讯作者：
Kocher TD