Genome-Wide Characterization of B-Box Gene Family and Its Roles in Responses to Light Quality and Cold Stress in Tomato.
Genome-Wide Characterization of B-Box Gene Family and Its Roles in Responses to Light Quality and Cold Stress in Tomato.
复制标题
B-Box 基因家族的全基因组特征及其在番茄光质和冷胁迫响应中的作用
DOI：
10.3389/fpls.2021.698525
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发表时间：
2021
影响因子：
5.6
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Bu X;Wang X;Yan J;Zhang Y;Zhou S;Sun X;Yang Y;Ahammed GJ;Liu Y;Qi M;Wang F;Li T
感知传入的环境信息对于优化植物生长和发育至关重要。多个B - box蛋白（BBXs）在植物依赖光的发育过程中发挥着重要作用。然而，BBXs在番茄植株中是否作为光和温度之间的信号整合因子仍不清楚。在本研究中，从新发布的番茄（Solanum lycopersicum）基因组序列中鉴定出31个SlBBX基因，并将它们聚类为5个亚组。基因结构和蛋白质基序分析表明，每个亚组内紧密聚类的SlBBX基因具有较高的保守性；然而，基因组定位分析显示SlBBX基因在番茄染色体上分布不均匀。启动子顺式调控元件预测和基因表达表明，SlBBX基因对光、激素和胁迫条件具有高度响应性。反向遗传学方法显示，SlBBX7、SlBBX9和SlBBX20的缺失在很大程度上抑制了番茄植株的耐寒性。此外，SlBBX7、SlBBX9和SlBBX20的损伤抑制了冷胁迫后立即出现的光合反应。由于非光化学猝灭（NPQ）受损，低温激发的过量光子能量和电子流在SlBBX7 -、SlBBX9 -和SlBBX20沉默的植株中未被消耗，导致电子载体过度还原和光系统受损。我们的研究强调了光信号转录因子SlBBXs在番茄植株耐寒性中的积极作用，这可能会增进对植物如何整合光和温度信号以适应不良环境的现有理解。
Perceiving incoming environmental information is critical for optimizing plant growth and development. Multiple B-box proteins (BBXs) play essential roles in light-dependent developmental processes in plants. However, whether BBXs function as a signal integrator between light and temperature in tomato plants remains elusive. In this study, 31 SlBBX genes were identified from the newly released tomato (Solanum lycopersicum) genome sequences and were clustered into five subgroups. Gene structure and protein motif analyses showed relatively high conservation of closely clustered SlBBX genes within each subgroup; however, genome mapping analysis indicated the uneven distribution of the SlBBX genes on tomato chromosomes. Promoter cis-regulatory elements prediction and gene expression indicated that SlBBX genes were highly responsive to light, hormones, and stress conditions. Reverse genetic approaches revealed that disruption of SlBBX7, SlBBX9, and SlBBX20 largely suppressed the cold tolerance of tomato plants. Furthermore, the impairment of SlBBX7, SlBBX9, and SlBBX20 suppressed the photosynthetic response immediately after cold stress. Due to the impairment of non-photochemical quenching (NPQ), the excess photon energy and electron flow excited by low temperature were not consumed in SlBBX7-, SlBBX9-, and SlBBX20- silenced plants, leading to the over reduction of electron carriers and damage of the photosystem. Our study emphasized the positive roles of light signaling transcription factors SlBBXs in cold tolerance in tomato plants, which may improve the current understanding of how plants integrate light and temperature signals to adapt to adverse environments.
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影响因子：
3.5
作者：
Crocco, Carlos D.;Botto, Javier F.
通讯作者：
Botto, Javier F.
番茄 B-BOX 基因家族的基因组组织、系统发育和表达分析。
影响因子：
5.6
作者：
Chu Z;Wang X;Li Y;Yu H;Li J;Lu Y;Li H;Ouyang B
通讯作者：
Ouyang B
影响因子：
8.8
作者：
Ding, Lan;Wang, Shuo;Liu, Jian-Xiang
通讯作者：
Liu, Jian-Xiang
影响因子：
5.2
作者：
Bai, Bo;Lu, Nannan;Xie, Xianzhi
通讯作者：
Xie, Xianzhi
影响因子：
7.4
作者：
Holtan, Hans E.;Bandong, Simona;Khanna, Rajnish
通讯作者：
Khanna, Rajnish