Genomic Organization, Phylogenetic and Expression Analysis of the B-BOX Gene Family in Tomato.
Genomic Organization, Phylogenetic and Expression Analysis of the B-BOX Gene Family in Tomato.
复制标题
番茄 B-BOX 基因家族的基因组组织、系统发育和表达分析。
DOI：
10.3389/fpls.2016.01552
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Ouyang B
中科院分区：
文献类型：
--
作者：
Chu Z;Wang X;Li Y;Yu H;Li J;Lu Y;Li H;Ouyang B
B-BOX （BBX）蛋白编码一类锌指转录因子，它们具有一个或两个B-BOX结构域，在某些情况下还具有CCT （CO， CO-like和TOC1）基序，在调节植物生长、发育和胁迫反应中发挥重要作用。然而，目前尚未对番茄（Solanum lycopersicum）中BBX基因进行系统的研究。在这里，我们提出了29个BBX基因的全基因组分析结果在这个重要的蔬菜物种。分析了它们的结构、保守域、系统发育关系、亚细胞定位和启动子顺式调控元件；研究了它们在不同激素和应激处理下的组织表达谱和表达模式。番茄BBX基因可分为5个亚家族，其中12个亚家族存在片段重复。实时荧光定量PCR分析显示，大部分BBX基因表现出不同的时空表达模式。干旱、聚乙二醇-6000或热胁迫均可诱导大部分BBX基因的表达。脱落酸、赤霉素、乙烯利等植物激素对部分BBX基因有较强的诱导作用。利用拟南芥叶肉原生质体进行的瞬时表达实验表明，7个BBX蛋白（SlBBX5、7、15、17、20、22和24）均定位于细胞核内。我们在基因组尺度上对番茄BBX基因的分析将为该家族特定基因的功能表征提供有价值的信息。
The B-BOX (BBX) proteins encode a class of zinc-finger transcription factors possessing one or two B-BOX domains and in some cases an additional CCT (CO, CO-like and TOC1) motif, which play important roles in regulating plant growth, development and stress response. Nevertheless, no systematic study of BBX genes has undertaken in tomato (Solanum lycopersicum). Here we present the results of a genome-wide analysis of the 29 BBX genes in this important vegetable species. Their structures, conserved domains, phylogenetic relationships, subcellular localizations, and promoter cis-regulatory elements were analyzed; their tissue expression profiles and expression patterns under various hormones and stress treatments were also investigated in detail. Tomato BBX genes can be divided into five subfamilies, and twelve of them were found to be segmentally duplicated. Real-time quantitative PCR analysis showed that most BBX genes exhibited different temporal and spatial expression patterns. The expression of most BBX genes can be induced by drought, polyethylene glycol-6000 or heat stress. Some BBX genes were induced strongly by phytohormones such as abscisic acid, gibberellic acid, or ethephon. The majority of tomato BBX proteins was predicted to be located in nuclei, and the transient expression assay using Arabidopsis mesophyll protoplasts demonstrated that all the seven BBX members tested (SlBBX5, 7, 15, 17, 20, 22, and 24) were localized in nucleus. Our analysis of tomato BBX genes on the genome scale would provide valuable information for future functional characterization of specific genes in this family.
登录
查看更多内容
影响因子：
3.5
作者：
Crocco, Carlos D.;Botto, Javier F.
通讯作者：
Botto, Javier F.
影响因子：
6.9
作者：
Gupta S;Shi X;Lindquist IE;Devitt N;Mudge J;Rashotte AM
通讯作者：
Rashotte AM
影响因子：
11.6
作者：
Datta, Sourav;Johansson, Henrik;Holm, Magnus
通讯作者：
Holm, Magnus
影响因子：
5.6
作者：
Diao WP;Snyder JC;Wang SB;Liu JB;Pan BG;Guo GJ;Wei G
通讯作者：
Wei G
影响因子：
11.6
作者：
Hirano, Ko;Nakajima, Masatoshi;Matsuoka, Makoto
通讯作者：
Matsuoka, Makoto