The Staphylococcus aureus Two-Component System AgrAC Displays Four Distinct Genomic Arrangements That Delineate Genomic Virulence Factor Signatures.
The Staphylococcus aureus Two-Component System AgrAC Displays Four Distinct Genomic Arrangements That Delineate Genomic Virulence Factor Signatures.
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DOI：
10.3389/fmicb.2018.01082
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发表时间：
2018
影响因子：
5.2
通讯作者：
Palsson BO
中科院分区：
文献类型：
--
作者：
Choudhary KS;Mih N;Monk J;Kavvas E;Yurkovich JT;Sakoulas G;Palsson BO
双组分系统(TCS)由一个组氨酸激酶和一个反应调节因子组成。在这里，我们评估了149个完全测序的金黄色葡萄球菌的AGAC TCS的保守性。它由四个基因组成：agrBDCA。我们发现：(1)除1株菌株外，其余所有菌株都存在agr系统(Agr)；(Ii)agr阳性菌株根据agr蛋白序列可进一步分为4种agr类型；(Iii)这4种agr类型不仅决定了agr基因的染色体排列，而且还决定了agr C组氨酸蛋白序列的差异，使其具有信号特异性；(Iv)序列差异反映在不同的结构特征上，特别是在跨膜区和第二细胞外结合区；(V)agr类型与生物体的毒力基因组图谱有很强的相关性。综上所述，这些结果表明，对agr基因座的生物信息学分析导致了一个与毒力因子和蛋白质结构特性相关的分类系统。
Two-component systems (TCSs) consist of a histidine kinase and a response regulator. Here, we evaluated the conservation of the AgrAC TCS among 149 completely sequenced Staphylococcus aureus strains. It is composed of four genes: agrBDCA. We found that: (i) AgrAC system (agr) was found in all but one of the 149 strains, (ii) the agr positive strains were further classified into four agr types based on AgrD protein sequences, (iii) the four agr types not only specified the chromosomal arrangement of the agr genes but also the sequence divergence of AgrC histidine kinase protein, which confers signal specificity, (iv) the sequence divergence was reflected in distinct structural properties especially in the transmembrane region and second extracellular binding domain, and (v) there was a strong correlation between the agr type and the virulence genomic profile of the organism. Taken together, these results demonstrate that bioinformatic analysis of the agr locus leads to a classification system that correlates with the presence of virulence factors and protein structural properties.
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影响因子：
5.6
作者：
Guerois, R;Nielsen, JE;Serrano, L
通讯作者：
Serrano, L
Biopython：用于计算分子生物学和生物信息学的免费 Python 工具。
DOI：
10.1093/bioinformatics/btp163
发表时间：
2009-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Cock PJ;Antao T;Chang JT;Chapman BA;Cox CJ;Dalke A;Friedberg I;Hamelryck T;Kauff F;Wilczynski B;de Hoon MJ
通讯作者：
de Hoon MJ
影响因子：
3.2
作者：
Dufour, P;Jarraud, S;Lina, G
通讯作者：
Lina, G
DOI：
10.1007/bf02191645
发表时间：
1995-08-30
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
NOVICK, RP;PROJAN, SJ;MOGHAZEH, S
通讯作者：
MOGHAZEH, S
影响因子：
3
作者：
Lim, King-Ting;Abu Hanifah, Yasmin;Thong, Kwai-Lin
通讯作者：
Thong, Kwai-Lin