The most common Chinese rhesus macaque MHC class I molecule shares peptide binding repertoire with the HLA-B7 supertype.
The most common Chinese rhesus macaque MHC class I molecule shares peptide binding repertoire with the HLA-B7 supertype.
复制标题
DOI：
10.1007/s00251-010-0450-3
复制
发表时间：
2010-07
期刊：
影响因子：
3.2
通讯作者：
Mothe, Bianca R.
中科院分区：
文献类型：
--
作者：
Solomon, Christopher;Southwood, Scott;Hoof, Ilka;Rudersdorf, Richard;Peters, Bjoern;Sidney, John;Pinilla, Clemencia;Marcondes, Maria Cecilia Garibaldi;Ling, Binhua;Marx, Preston;Sette, Alessandro;Mothe, Bianca R.
在用于艾滋病研究的两个恒河猴亚种中，感染猿猴免疫缺陷病毒的印度恒河猴（Macaca mulatta）是最成熟的HIV感染模型，它既提供了对发病机制的深入了解，也提供了测试新型疫苗的系统。尽管中国恒河猴可能是一个更相关的模型，艾滋病的结果比印度恒河猴，中国起源的恒河猴还没有很好地表征其主要组织相容性复合体（MHC）的组成和功能，减少其更大的利用。在这项研究中，我们的特点，共50个独特的中国恒河猴从几个不同的起源，他们的整个MHC I类等位基因组成，并确定了58个独特的完整的MHC I类序列。其中只有9个序列与印度恒河猴有关，并且确定的序列中有28/58（48.3%）是新序列。从检测到的所有MHC等位基因中，我们基于Mamu-A1*02201较高的表达频率优先进行功能表征。在MHC/肽结合试验的发展及其相关基序的定义，我们发现，该等位基因的HLA-B7超型，在人群中最常见的超型共享肽结合特性。这些研究提供了第一个功能特性的MHC I类分子的背景下，中国恒河猴和第一个实例的HLA-B7恒河猴的类比。本文的在线版本（doi：10.1007/s 00251 -010-0450-3）包含补充材料，可供授权用户使用。
Of the two rhesus macaque subspecies used for AIDS studies, the Simian immunodeficiency virus-infected Indian rhesus macaque (Macaca mulatta) is the most established model of HIV infection, providing both insight into pathogenesis and a system for testing novel vaccines. Despite the Chinese rhesus macaque potentially being a more relevant model for AIDS outcomes than the Indian rhesus macaque, the Chinese-origin rhesus macaques have not been well-characterized for their major histocompatibility complex (MHC) composition and function, reducing their greater utilization. In this study, we characterized a total of 50 unique Chinese rhesus macaques from several varying origins for their entire MHC class I allele composition and identified a total of 58 unique complete MHC class I sequences. Only nine of the sequences had been associated with Indian rhesus macaques, and 28/58 (48.3%) of the sequences identified were novel. From all MHC alleles detected, we prioritized Mamu-A1*02201 for functional characterization based on its higher frequency of expression. Upon the development of MHC/peptide binding assays and definition of its associated motif, we revealed that this allele shares peptide binding characteristics with the HLA-B7 supertype, the most frequent supertype in human populations. These studies provide the first functional characterization of an MHC class I molecule in the context of Chinese rhesus macaques and the first instance of HLA-B7 analogy for rhesus macaques. The online version of this article (doi:10.1007/s00251-010-0450-3) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
5.4
作者：
Loffredo, John T.;Maxwell, Jess;Watkins, David I.
通讯作者：
Watkins, David I.
影响因子：
--
作者：
Knapp, LA;Lehmann, E;Watkins, DI
通讯作者：
Watkins, DI
影响因子：
5.6
作者：
Gulukota, K;Sidney, J;DeLisi, C
通讯作者：
DeLisi, C
影响因子：
6.4
作者：
Brander, C;O'Connor, P;Scadden, DT
通讯作者：
Scadden, DT
影响因子：
3.8
作者：
Burdo, TH;Marcondes, MCG;Fox, HS
通讯作者：
Fox, HS