Bombyx mori Nucleopolyhedrovirus (BmNPV) Induces G2/M Arrest to Promote Viral Multiplication by Depleting BmCDK1.
Bombyx mori Nucleopolyhedrovirus (BmNPV) Induces G2/M Arrest to Promote Viral Multiplication by Depleting BmCDK1.
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家蚕核多角体病毒 (BmNPV) 通过消耗 BmCDK1 诱导 G2/M 阻滞以促进病毒增殖
DOI：
10.3390/insects12121098
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发表时间：
2021-12-08
期刊：
影响因子：
3
通讯作者：
Pan MH
中科院分区：
文献类型：
--
作者：
Xiao Q;Dong ZQ;Zhu Y;Zhang Q;Yang X;Xiao M;Chen P;Lu C;Pan MH
杆状病毒可使昆虫细胞的细胞周期停滞在S期或G2/M期，但其确切机制尚不清楚。家蚕核型多角体病毒（BmNPV）是家蚕的重要病原，是一种特征性最强的杆状病毒。在本研究中，我们确定BmCDK 1和BmCyclin B表达下调是BmNPV介导的G2/M期阻滞所必需的，这在促进BmNPV复制中起着重要作用。进一步的研究表明BmNPV IAP 1与BmCDK 1相互作用。BmNPViap 1基因的过表达导致细胞聚集在G2/M期，而BmNPViap 1基因的敲除减弱了BmNPViap 1基因介导的G2/M期阻滞效应。这些发现增强了对BmNPV发病机制的理解，并表明杆状病毒影响细胞周期进程的新机制。摘要了解病毒与宿主的相互作用对于阐明病毒复制和宿主抗性的机制具有重要意义。杆状病毒是一种昆虫病毒，可引起昆虫细胞S期或G2/M期阻滞。然而，杆状病毒介导的S或G2/M期阻滞的作用和机制尚未完全了解。利用流式细胞术（FCM）、微管蛋白标记、BrdU标记和CellTiter 96® AQueous One Solution Cell Proliferation Assay（MTS）获得的结果表明，家蚕核型多角体病毒（BmNPV）诱导BmN-SWU 1细胞的G2/M期停滞，并抑制细胞DNA复制以及细胞增殖。我们发现BmNPV通过降低BmCDK 1和BmCyclin B的表达，诱导G2/M期阻滞，以支持其复制和增殖。免疫共沉淀实验证实BmNPV IAP 1与BmCDK 1相互作用。BmNPViap 1通过降低BmCDK 1含量参与BmNPVG 2/M期阻滞过程。总之，我们的研究结果提高了对病毒-宿主相互作用网络的理解，并提供了一个潜在的靶基因，连接细胞凋亡和细胞周期。
Simple Summary Baculoviruses arrest the cell cycle in the S or G2/M phase in insect cells, but the exact mechanism of this process still remains obscure. Bombyx mori nucleopolyhedrovirus (BmNPV), one of the best characterized baculoviruses, is an important pathogen in silkworms. In the present study, we determined that downregulation of BmCDK1 and BmCyclin B expression was required for BmNPV-mediated G2/M phase arrest, which plays an essential role in facilitating BmNPV replication. Further investigations showed that BmNPV IAP1 interacted with BmCDK1. The overexpression of the BmNPV iap1 gene led to the accumulation of cells in the G2/M phase, and BmNPV iap1 gene knockdown attenuated the effect of BmNPV-mediated G2/M phase arrest. These findings enhance the understanding of BmNPV pathogenesis, and indicate a novel mechanism through which baculoviruses impact the cell cycle progression. Abstract Understanding virus–host interaction is very important for delineating the mechanism involved in viral replication and host resistance. Baculovirus, an insect virus, can cause S or G2/M phase arrest in insect cells. However, the roles and mechanism of Baculovirus-mediated S or G2/M phase arrest are not fully understood. Our results, obtained using flow cytometry (FCM), tubulin-labeling, BrdU-labeling, and CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS), showed that Bombyx mori nucleopolyhedrovirus (BmNPV) induced G2/M phase arrest and inhibited cellular DNA replication as well as cell proliferation in BmN-SWU1 cells. We found that BmNPV induced G2/M arrest to support its replication and proliferation by reducing the expression of BmCDK1 and BmCyclin B. Co-immunoprecipitation assays confirmed that BmNPV IAP1 interacted with BmCDK1. BmNPV iap1 was involved in the process of BmNPV-induced G2/M arrest by reducing the content of BmCDK1. Taken together, our results improve the understanding of the virus–host interaction network, and provide a potential target gene that connects apoptosis and the cell cycle.
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DOI：
10.1074/jbc.m117.776112
发表时间：
2017-03-24
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fan Y;Mok CK;Chan MC;Zhang Y;Nal B;Kien F;Bruzzone R;Sanyal S
通讯作者：
Sanyal S
影响因子：
5.4
作者：
Chen, CJ;Makino, S
通讯作者：
Makino, S
BmNPV感染后易感和抗性家蚕品系选择性剪接差异的全转录组分析
影响因子：
2.8
作者：
Li, Gang;Zhou, Kaiyue;Xu, Anying
通讯作者：
Xu, Anying
影响因子：
5.4
作者：
Dove, B;Brooks, G;Hiscox, JA
通讯作者：
Hiscox, JA
影响因子：
64.8
作者：
LEE, MG;NORBURY, CJ;NURSE, P
通讯作者：
NURSE, P