Bioinformatic Analysis of the Proteome in Exosomes Derived From Plasma: Exosomes Involved in Cholesterol Metabolism Process of Patients With Spinal Cord Injury in the Acute Phase.
Bioinformatic Analysis of the Proteome in Exosomes Derived From Plasma: Exosomes Involved in Cholesterol Metabolism Process of Patients With Spinal Cord Injury in the Acute Phase.
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血浆外泌体蛋白质组生物信息学分析：外泌体参与脊髓损伤急性期患者胆固醇代谢过程
DOI：
10.3389/fninf.2021.662967
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发表时间：
2021
影响因子：
3.5
通讯作者：
Cui Z
中科院分区：
文献类型：
--
作者：
Wu C;Yu J;Xu G;Gao H;Sun Y;Huang J;Sun L;Zhang X;Cui Z
脊髓损伤(SCI)是交通事故引起的一种常见病、重病。由血脂异常引起的冠状动脉粥样硬化性心脏病(CHD)是脊髓损伤患者的主要死亡原因。定量分析显示，脊髓损伤后48h内，外周血中胆固醇和脂蛋白浓度无明显变化。由于血脊髓屏障(BSCB)的存在，有关SCI急性期血浆胆固醇代谢的研究很少。胞外体具有较小的颗粒尺寸，这使得它们能够相对较少地受到BSCB的限制。本研究以43例脊髓损伤急性期患者和71例对照组患者的血浆外切体为样本。MS蛋白质组学和生物信息学分析发现了590个可定量蛋白质，其中75个蛋白质上调，153个蛋白质下调，前10个差异表达蛋白质包括下调蛋白：HIST1H4A、HIST2H3A、HIST2H2BE、HCLS1、S100A9、HIST1H2 BM、S100A8、CALM3、YWHAH和SFN，以及上调蛋白：SERPIND1、C1QB、SPTLC3、IGHV4-28、C4a、IGHV4-38-2、IGHV4-30-2、SLC15A1、C4b和ACTG2。浓缩分析表明，在下调的蛋白质中，与胆固醇代谢相关的蛋白质比例最大。患者血浆外周血中胆固醇的主要成分[ApoB-48和ApoB-100升高，ApoA-I、ApoA-II、ApoA-IV、ApoC、ApoE和ApoA(A)降低]发生变化。ELISA法分析显示，脊髓损伤急性期部分成分紊乱。提示外切体可能参与了脊髓损伤急性期的胆固醇代谢调节。
Spinal cord injury (SCI) is a common but severe disease caused by traffic accidents. Coronary atherosclerotic heart disease (CHD) caused by dyslipidemia is known as the leading cause of death in patients with SCI. However, the quantitative analysis showed that the cholesterol and lipoprotein concentrations in peripheral blood (PB) did not change significantly within 48 h after SCI. Due to the presence of the Blood spinal cord barrier (BSCB), there are only few studies concerning the plasma cholesterol metabolism in the acute phase of SCI. Exosomes have a smaller particle size, which enables them relatively less limitation of BSCB. This study uses exosomes derived from the plasma of 43 patients in the acute phase of SCI and 71 patients in the control group as samples. MS proteomics and bioinformatics analysis found 590 quantifiable proteins, in which 75 proteins were upregulated and 153 proteins were downregulated, and the top 10 differentially expressed proteins are those including downregulating proteins: HIST1H4A, HIST2H3A, HIST2H2BE, HCLS1, S100A9, HIST1H2BM, S100A8, CALM3, YWHAH, and SFN, and upregulating proteins: SERPIND1, C1QB, SPTLC3, IGHV4-28, C4A, IGHV4-38-2, IGHV4-30-2, SLC15A1, C4B, and ACTG2. Enrichment analysis showed that the largest part of proteins was related to cholesterol metabolism among the downregulated proteins. The main components of cholesterol [ApoB-48 and ApoB-100 increased, ApoA-I, ApoA-II, ApoA-IV, ApoC, ApoE, and Apo(a) decreased] were changed in exosomes derived from plasma of patients. ELISA analysis showed that some components were disordered in the acute phase of SCI. These results suggested that the exosomes might be involved in cholesterol metabolism regulation in the acute phase of SCI.
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影响因子：
16
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
通讯作者：
Théry C
影响因子：
3
作者：
Ahadi, Reza;Khodagholi, Fariba;Jorjani, Masoumeh
通讯作者：
Jorjani, Masoumeh
DOI：
10.1016/j.apmr.2014.10.021
发表时间：
2015-04-01
影响因子：
4.3
作者：
Bank, Matthew;Stein, Adam;Bloom, Ona
通讯作者：
Bloom, Ona
影响因子：
3
作者：
Casper, David S.;Zmistowski, Benjamin;Kepler, Christopher K.
通讯作者：
Kepler, Christopher K.
影响因子：
2.5
作者：
Lee DY;Park YJ;Song SY;Hwang SC;Kim KT;Kim DH
通讯作者：
Kim DH