Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo.
Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo.
复制标题
在体内，Rab11内切小体和周丝蛋白协调着脱落前中心体的运动。
DOI：
10.26508/lsa.202201362
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发表时间：
2022-07
影响因子：
4.4
通讯作者：
Hehnly H
中科院分区：
文献类型：
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作者：
Krishnan N;Swoger M;Rathbun LI;Fioramonti PJ;Freshour J;Bates M;Patteson AE;Hehnly H
当两个子细胞将它们最老的中心体向细胞动力学桥移动时，细胞分裂完成，然后在脱落过程中分裂。GTPase （Rab11）和中心体蛋白（Pericentrin）共同协调这一运动。细胞分裂的最后阶段包括两个子细胞通过细胞动力学桥保持相互连接，该桥在脱落过程中断裂。在斑马鱼胚胎和人类细胞之间，我们发现最老的中心体以依赖rab11的方式向细胞动力学桥移动，有时最年轻的中心体紧随其后。在分离前，Rab11-核内体在母中心粒以依赖于Rab11- gtp的方式组织，在最老的中心粒上的Rab11核内体比最年轻的中心粒更具流动性。Rab11的GTPase活性是中心体蛋白（Pericentrin）在中心体富集所必需的。周心蛋白表达的减少或rab11 -核内体功能的光遗传破坏抑制了中心体向细胞动力学桥的运动和脱落，导致子细胞容易形成双核和/或有多余的中心体。这些研究表明，rab11 -核内体通过调节中心蛋白组织，导致中心体向细胞动力学桥的适当运动和随后的脱落，从而有助于中心体在脱落前的功能。
Cell division completes when the two daughter cells move their oldest centrosome towards the cytokinetic bridge, which is then cleaved during abscission. The GTPase, Rab11, and the centrosome protein, Pericentrin, work together to coordinate this movement. The last stage of cell division involves two daughter cells remaining interconnected by a cytokinetic bridge that is cleaved during abscission. Conserved between the zebrafish embryo and human cells, we found that the oldest centrosome moves in a Rab11-dependent manner towards the cytokinetic bridge sometimes followed by the youngest. Rab11-endosomes are organized in a Rab11-GTP dependent manner at the mother centriole during pre-abscission, with Rab11 endosomes at the oldest centrosome being more mobile compared with the youngest. The GTPase activity of Rab11 is necessary for the centrosome protein, Pericentrin, to be enriched at the centrosome. Reduction in Pericentrin expression or optogenetic disruption of Rab11-endosome function inhibited both centrosome movement towards the cytokinetic bridge and abscission, resulting in daughter cells prone to being binucleated and/or having supernumerary centrosomes. These studies suggest that Rab11-endosomes contribute to centrosome function during pre-abscission by regulating Pericentrin organization resulting in appropriate centrosome movement towards the cytokinetic bridge and subsequent abscission.
DOI：
10.1038/nrm3395
发表时间：
2012-07-11
期刊：
Nature reviews. Molecular cell biology
影响因子：
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作者：
通讯作者：
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