Monitoring Cu2+‐Binding to a DNA‐Clip‐phen Conjugate and Metal‐centered Redox Processes by a Fluorescent Reporter Group

Monitoring Cu2+‐Binding to a DNA‐Clip‐phen Conjugate and Metal‐centered Redox Processes by a Fluorescent Reporter Group
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通过荧光报告基团监测 Cu2+-与 DNA-Clip-phen 缀合物的结合和以金属为中心的氧化还原过程

DOI:
10.1002/zaac.201300077
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发表时间:
2013
期刊:
Zeitschrift für anorganische und allgemeine Chemie
影响因子:
--
通讯作者:
R. Krämer
R. Krämer
中科院分区:
--
文献类型:
--
作者:
Arndt Sprödefeld;A. Kiel;D. Herten;R. Krämer

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制备了双-1,10-菲咯啉配体的DNA偶联物CLIP-phen,并将其与互补的荧光报告标记的DNA链结合。在得到的DS-DNA中,荧光报告既发出Cu2+离子与Clip-phen部分结合的信号,也发出通过改变荧光强度还原为Cu+的信号。在初步的单分子荧光研究中,这些过程似乎可以通过单分子荧光光谱在单个分子的水平上观察到,但需要进一步验证和优化该系统。
A DNA conjugate of the bis-1,10-phenanthroline ligand Clip-phen was prepared and combined with a complementary, fluorescent reporter labeled DNA strand. In the resulting ds-DNA, the fluorescent reporter signals both the binding of Cu2+ ions to the Clip-phen moiety and the reduction to Cu+ by a change in fluorescence intensity. In preliminary single molecule fluorescence investigations, these processes appear to be observable on the level of individual molecules by single molecule fluorescence spectroscopy, but further validation and optimisation of the system is needed.
DOI: 10.1021/ja307276z
发表时间: 2012-10-03
影响因子: 15
作者:
Chacón KN;Blackburn NJ
通讯作者: Blackburn NJ
DOI: 10.1016/j.abb.2004.11.025
发表时间: 2005
期刊: Archives of biochemistry and biophysics.
影响因子: --
作者:
Mandal,Subrata;Kazmi,NajamH;Sayre,LawrenceM
通讯作者: Sayre,LawrenceM