Monitoring Cu2+‐Binding to a DNA‐Clip‐phen Conjugate and Metal‐centered Redox Processes by a Fluorescent Reporter Group
Monitoring Cu2+‐Binding to a DNA‐Clip‐phen Conjugate and Metal‐centered Redox Processes by a Fluorescent Reporter Group
复制标题
通过荧光报告基团监测 Cu2+-与 DNA-Clip-phen 缀合物的结合和以金属为中心的氧化还原过程
DOI:
10.1002/zaac.201300077
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发表时间:
2013
期刊:
影响因子:
--
通讯作者:
R. Krämer
中科院分区:
文献类型:
--
作者:
Arndt Sprödefeld;A. Kiel;D. Herten;R. Krämer
A DNA conjugate of the bis-1,10-phenanthroline ligand Clip-phen was prepared and combined with a complementary, fluorescent reporter labeled DNA strand. In the resulting ds-DNA, the fluorescent reporter signals both the binding of Cu2+ ions to the Clip-phen moiety and the reduction to Cu+ by a change in fluorescence intensity. In preliminary single molecule fluorescence investigations, these processes appear to be observable on the level of individual molecules by single molecule fluorescence spectroscopy, but further validation and optimisation of the system is needed.
影响因子:
15
作者:
Chacón KN;Blackburn NJ
通讯作者:
Blackburn NJ
DOI:
10.1016/j.abb.2004.11.025
发表时间:
2005
期刊:
Archives of biochemistry and biophysics.
影响因子:
--
作者:
Mandal,Subrata;Kazmi,NajamH;Sayre,LawrenceM
通讯作者:
Sayre,LawrenceM