The CCCTC-binding factor (CTCF) of Drosophila contributes to the regulation of the ribosomal DNA and nucleolar stability.
The CCCTC-binding factor (CTCF) of Drosophila contributes to the regulation of the ribosomal DNA and nucleolar stability.
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DOI：
10.1371/journal.pone.0016401
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发表时间：
2011-01-20
期刊：
影响因子：
3.7
通讯作者：
Maggert KA
中科院分区：
文献类型：
--
作者：
Guerrero PA;Maggert KA
在核糖体 DNA (rDNA) 的重复序列中，只有大约一半的基因活跃转录，而其他基因则沉默。在节肢动物中，转座元件会中断一部分基因，通常会使这些基因的转录失活。尽管启动子序列相同，但关于并置的活性和非活性染色质结构域的建立或分离，或转座元件中断基因的优先失活，人们知之甚少。 CTCF 是一种序列特异性 DNA 结合蛋白，被认为可充当转录阻遏蛋白，阻断增强子-启动子通讯，并界定活性和非活性染色质的并置结构域；这些活动中的一种或多种可能有助于调节该重复基因簇。为了支持这一假设，我们证明果蝇核仁含有 CTCF，它与 rDNA 内的转座元件序列结合。 CTCF 基因活性的降低会导致核仁断裂并减少 rDNA 沉默，而多聚 ADP 核糖基化的破坏被认为是 CTCF 核仁定位所必需的。我们的数据确定了 CTCF 作为正确控制转座元件负载的 rDNA 转录和核仁稳定性所必需的成分的作用。
In the repeat array of ribosomal DNA (rDNA), only about half of the genes are actively transcribed while the others are silenced. In arthropods, transposable elements interrupt a subset of genes, often inactivating transcription of those genes. Little is known about the establishment or separation of juxtaposed active and inactive chromatin domains, or preferential inactivation of transposable element interrupted genes, despite identity in promoter sequences. CTCF is a sequence-specific DNA binding protein which is thought to act as a transcriptional repressor, block enhancer-promoter communication, and delimit juxtaposed domains of active and inactive chromatin; one or more of these activities might contribute to the regulation of this repeated gene cluster. In support of this hypothesis, we show that the Drosophila nucleolus contains CTCF, which is bound to transposable element sequences within the rDNA. Reduction in CTCF gene activity results in nucleolar fragmentation and reduced rDNA silencing, as does disruption of poly-ADP-ribosylation thought to be necessary for CTCF nucleolar localization. Our data establish a role for CTCF as a component necessary for proper control of transposable element-laden rDNA transcription and nucleolar stability.
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