Phenotypic and Functional Characteristics of Exosomes Derived from Irradiated Mouse Organs and Their Role in the Mechanisms Driving Non-Targeted Effects.
Phenotypic and Functional Characteristics of Exosomes Derived from Irradiated Mouse Organs and Their Role in the Mechanisms Driving Non-Targeted Effects.
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DOI：
10.3390/ijms21218389
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发表时间：
2020-11-09
影响因子：
5.6
通讯作者：
Kadhim M
中科院分区：
文献类型：
--
作者：
Tuncay Cagatay S;Mayah A;Mancuso M;Giardullo P;Pazzaglia S;Saran A;Daniel A;Traynor D;Meade AD;Lyng F;Tapio S;Kadhim M
受辐射和未受辐射的相邻细胞之间的分子通讯引发辐射诱导的旁观者效应（里贝）和场外（远场）效应，这两者都是电离辐射（IR）的非靶向效应（NTE）的一个实例。外泌体是内体起源的小膜囊泡和新鉴定的NTE介质。虽然外泌体介导的变化是有据可查的放射治疗和肿瘤学，有一个缺乏知识的作用外泌体来自内部和外部的辐射领域的早期和延迟诱导NTE后IR。因此，在这里，我们调查的变化外泌体的配置文件和外泌体作为可能的分子信号传导介质的辐射损伤。将来自全身照射（WBI）或部分身体照射（PBI）小鼠的器官的外泌体在照射后24小时和15天转移到受体小鼠胚胎成纤维细胞（MEF）细胞中，并与用来自未照射小鼠的外泌体处理的MEF细胞相比，评价细胞活力、DNA损伤和钙、活性氧和一氧化氮信号传导的变化。总之，我们的研究结果表明，全身和局部照射增加了外泌体的数量，引起了外泌体处理的MEF细胞的变化，这取决于暴露后的来源器官和时间。
Molecular communication between irradiated and unirradiated neighbouring cells initiates radiation-induced bystander effects (RIBE) and out-of-field (abscopal) effects which are both an example of the non-targeted effects (NTE) of ionising radiation (IR). Exosomes are small membrane vesicles of endosomal origin and newly identified mediators of NTE. Although exosome-mediated changes are well documented in radiation therapy and oncology, there is a lack of knowledge regarding the role of exosomes derived from inside and outside the radiation field in the early and delayed induction of NTE following IR. Therefore, here we investigated the changes in exosome profile and the role of exosomes as possible molecular signalling mediators of radiation damage. Exosomes derived from organs of whole body irradiated (WBI) or partial body irradiated (PBI) mice after 24 h and 15 days post-irradiation were transferred to recipient mouse embryonic fibroblast (MEF) cells and changes in cellular viability, DNA damage and calcium, reactive oxygen species and nitric oxide signalling were evaluated compared to that of MEF cells treated with exosomes derived from unirradiated mice. Taken together, our results show that whole and partial-body irradiation increases the number of exosomes, instigating changes in exosome-treated MEF cells, depending on the source organ and time after exposure.
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影响因子：
5.6
作者：
Jabbari, Nasrollah;Nawaz, Muhammad;Rezaie, Jafar
通讯作者：
Rezaie, Jafar
影响因子：
64.5
作者：
Boelens MC;Wu TJ;Nabet BY;Xu B;Qiu Y;Yoon T;Azzam DJ;Twyman-Saint Victor C;Wiemann BZ;Ishwaran H;Ter Brugge PJ;Jonkers J;Slingerland J;Minn AJ
通讯作者：
Minn AJ
影响因子：
1.7
作者：
Jelonek, Karol;Wojakowska, Anna;Pietrowska, Monika
通讯作者：
Pietrowska, Monika
影响因子：
6
作者：
Bolmont, Tristan;Clavaguera, Florence;Jucker, Mathias
通讯作者：
Jucker, Mathias
影响因子：
3.7
作者：
Chandna, S
通讯作者：
Chandna, S