Genome-wide copy number profiling to detect gene amplifications in neural progenitor cells
Genome-wide copy number profiling to detect gene amplifications in neural progenitor cells
复制标题
全基因组拷贝数分析以检测神经祖细胞中的基因扩增
DOI：
10.1016/j.gdata.2014.06.020
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Meese E
中科院分区：
文献类型：
--
作者：
Fischer U;Keller A;Backes C;Meese E
DNA 序列扩增发生在两栖动物和果蝇正常发育过程中的特定阶段，而在人类中似乎仅限于耐药细胞和肿瘤细胞。我们使用 array-CGH 来发现人类神经祖细胞分化过程中的拷贝数变化，包括基因扩增和缺失。在这里，我们描述了为识别基因组拷贝数变化而定制的细胞培养特征、DNA 提取和比较基因组杂交 (CGH) 分析。 Fischer 及其同事于 2012 年在 PLOS One 上发表了与该实验相关的扩增染色体区域的进一步详细分析（Fischer et al., 2012）。我们提供了分化过程中删除的染色体区域的详细信息，并概述了两个代表性染色体区域在分化诱导过程中的拷贝数变化。
DNA sequence amplification occurs at defined stages during normal development in amphibians and flies and seems to be restricted in humans to drug-resistant and tumor cells only. We used array-CGH to discover copy number changes including gene amplifications and deletions during differentiation of human neural progenitor cells. Here, we describe cell culture features, DNA extraction, and comparative genomic hybridization (CGH) analysis tailored towards the identification of genomic copy number changes. Further detailed analysis of amplified chromosome regions associated with this experiment, was published by Fischer and colleagues in PLOS One in 2012 (Fischer et al., 2012). We provide detailed information on deleted chromosome regions during differentiation and give an overview on copy number changes during differentiation induction for two representative chromosome regions.
影响因子：
3.7
作者：
Fischer U;Keller A;Voss M;Backes C;Welter C;Meese E
通讯作者：
Meese E