Effect of bio-engineering on size, shape, composition and rigidity of bacterial microcompartments.
Effect of bio-engineering on size, shape, composition and rigidity of bacterial microcompartments.
复制标题
生物工程对细菌微剖间的大小，形状，组成和刚性的影响。
DOI：
10.1038/srep36899
复制
发表时间：
2016-11-15
影响因子：
4.6
通讯作者：
Warren MJ
中科院分区：
文献类型：
--
作者：
Mayer MJ;Juodeikis R;Brown IR;Frank S;Palmer DJ;Deery E;Beal DM;Xue WF;Warren MJ
细菌微区室（BMC）是广泛存在于细菌中的蛋白质细胞器，由外壳组成，外壳包裹代表特定代谢过程的酶货物。外壳由许多不同的蛋白质组成，这些蛋白质形成六聚体和五聚体瓦片，这些瓦片相互作用以形成多面体建筑物。我们先前已经表明，与1，2-丙二醇利用相关的弗氏柠檬酸杆菌BMC可以转移到大肠杆菌中以产生重组BMC，并且空BMC可以仅由壳蛋白单独形成。在本文中，通过2D-凝胶电泳、质谱、透射电子显微镜（TEM）和原子力显微镜（AFM）将野生型BMC的详细结构和蛋白质组学表征与重组BMC和许多空BMC变体进行比较。具体地，显示野生型BMC和重组BMC在组成、大小、形状和机械性质方面是相似的，而空BMC变体显示为更小、中空且延展性更低。
Bacterial microcompartments (BMCs) are proteinaceous organelles that are found in a broad range of bacteria and are composed of an outer shell that encases an enzyme cargo representing a specific metabolic process. The outer shell is made from a number of different proteins that form hexameric and pentameric tiles, which interact to allow the formation of a polyhedral edifice. We have previously shown that the Citrobacter freundii BMC associated with 1,2-propanediol utilization can be transferred into Escherichia coli to generate a recombinant BMC and that empty BMCs can be formed from just the shell proteins alone. Herein, a detailed structural and proteomic characterization of the wild type BMC is compared to the recombinant BMC and a number of empty BMC variants by 2D-gel electrophoresis, mass spectrometry, transmission electron microscopy (TEM) and atomic force microscopy (AFM). Specifically, it is shown that the wild type BMC and the recombinant BMC are similar in terms of composition, size, shape and mechanical properties, whereas the empty BMC variants are shown to be smaller, hollow and less malleable.
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影响因子：
3.2
作者：
Johnson, CLV;Pechonick, E;Bobik, TA
通讯作者：
Bobik, TA
DOI：
10.1073/pnas.1423672112
发表时间：
2015-03-10
影响因子：
11.1
作者：
Chowdhury, Chiranjit;Chun, Sunny;Bobik, Thomas A.
通讯作者：
Bobik, Thomas A.
影响因子：
56.9
作者：
Kerfeld, CA;Sawaya, MR;Yeates, TO
通讯作者：
Yeates, TO
影响因子：
2.2
作者：
Fox, Karen;Fox, Alvin;Walla, Michael
通讯作者：
Walla, Michael
影响因子：
3.2
作者：
Bobik, TA;Havemann, GD;Aldrich, HC
通讯作者：
Aldrich, HC