OsRAD17 Is Required for Meiotic Double-Strand Break Repair and Plays a Redundant Role With OsZIP4 in Synaptonemal Complex Assembly.
OsRAD17 Is Required for Meiotic Double-Strand Break Repair and Plays a Redundant Role With OsZIP4 in Synaptonemal Complex Assembly.
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OsRAD17 是减数分裂双链断裂修复所必需的，并在联会复合体组装中与 OsZIP4 一起发挥冗余作用
DOI：
10.3389/fpls.2018.01236
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发表时间：
2018
影响因子：
5.6
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Hu Q;Zhang C;Xue Z;Ma L;Liu W;Shen Y;Ma B;Cheng Z
同源重组(HR)修复依赖于SPO11的双链断裂(DSB)确保了同源染色体的正确分离。在酵母和人类中，RAD17参与DNA损伤检查点控制和DSB修复。然而，人们对其在植物中的作用知之甚少。在这项研究中，我们鉴定了水稻中的RAD17同源基因。在Osrad17花粉母细胞(PMC)中，经常观察到非同源染色体之间的关联和染色体碎裂。这些异常的染色体关联依赖于程序化DSB的形成。OsRAD17与OsRAD1相互作用，Osrad1和Osrad17的减数分裂表型与表型相似的两个单一突变体没有区别，表明它们可能在同一条途径上起作用。OsZIP4、OsMSH5和OsMER3是水稻ZMM蛋白的成员，它们是杂交形成所必需的。我们发现，在Oszip4和Osrad17单突变体中，同源配对和突触几乎没有受到影响，而在Oszip4和Osrad17双突变体中，同源配对和突触受到严重干扰。在Osmsh5 Osrad17和Osmer3 Osrad1双突变体中观察到相似的表型，这表明Checkpoint蛋白和ZMM蛋白在确保水稻准确的HR方面具有协同作用。
The repair of SPO11-dependent double-strand breaks (DSBs) by homologous recombination (HR) ensures the correct segregation of homologous chromosomes. In yeast and human, RAD17 is involved in DNA damage checkpoint control and DSB repair. However, little is known about its function in plants. In this study, we characterized the RAD17 homolog in rice. In Osrad17 pollen mother cells (PMCs), associations between non-homologous chromosomes and chromosome fragmentation were constantly observed. These aberrant chromosome associations were dependent on the formation of programmed DSBs. OsRAD17 interacts with OsRAD1 and the meiotic phenotype of Osrad1 Osrad17 is indistinguishable from the two single mutants which have similar phenotypes, manifesting they could act in the same pathway. OsZIP4, OsMSH5 and OsMER3 are members of ZMM proteins in rice that are required for crossover formation. We found that homologous pairing and synapsis, which was roughly unaffected in Oszip4 and Osrad17 single mutant, was severely disturbed in the Oszip4 Osrad17 double mutant. Similar phenotypes were observed in the Osmsh5 Osrad17 and Osmer3 Osrad1 double mutants, suggesting the cooperation between the checkpoint proteins and ZMM proteins in assuring accurate HR in rice.
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影响因子：
4.8
作者：
Griffith, JD;Lindsey-Boltz, LA;Sancar, A
通讯作者：
Sancar, A
影响因子：
5.6
作者：
Lambing C;Heckmann S
通讯作者：
Heckmann S
影响因子：
7.2
作者：
Heitzeberg, F;Chen, IP;Puchta, H
通讯作者：
Puchta, H
影响因子：
4.5
作者：
Girard C;Chelysheva L;Choinard S;Froger N;Macaisne N;Lemhemdi A;Mazel J;Crismani W;Mercier R
通讯作者：
Mercier R
影响因子：
3.8
作者：
Navadgi-Patil VM;Burgers PM
通讯作者：
Burgers PM