Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish
Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish
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抗菌肽 (AMP) 的表达谱及其 Relish 的调节
DOI：
10.1007/s00343-012-1195-7
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发表时间：
2012-07
影响因子：
--
通讯作者：
Jianhai Xiang
中科院分区：
文献类型：
--
作者：
Dongdong Wang;Fuhua Li;Shihao Li;Rong Wen;Jianhai Xiang
抗菌肽作为关键的免疫效应物，在无脊椎动物的天然免疫系统中发挥着重要作用。在不同对虾中已经发现了不同类型的AMPs，包括对虾蛋白、Crustin、ALF(抗脂多糖因子)，但对不同类型的AMPs在对虾中对不同类型细菌的响应功能的系统分析非常有限。在本研究中，我们同时用实时定量RT-PCR法分析了中国对虾感染溶血性微球菌(革兰氏阳性，G+)和鳗弧菌(革兰氏阴性，G-−)时AMPs的表达谱。不同的AMP在对虾注射同一种细菌时表现出不同的表达谱，同一种AMP在对虾受到不同的细菌攻击时也表现出不同的表达谱。此外，这些AMPs的表达呈现时间表达谱，表明不同的AMPs在细菌感染的对虾中协同发挥作用。采用RNA干扰的方法研究了味觉转录因子在调节不同AMP转录中的功能。目前的研究表明，调味料可以调节对虾不同AMP的转录。对虾注射不同类型细菌后AMPs的差异表达谱表明，对虾体内存在着复杂的抗菌反应网络。这些数据有助于我们了解对虾的免疫功能，并可能为对虾疾病的控制提供策略。
Antimicrobial peptides (AMPs), as key immune effectors, play important roles in the innate immune system of invertebrates. Different types of AMPs, including Penaeidin, Crustin, ALF (antilipopolysaccharide factor) have been identified in different penaeid shrimp; however, systematic analyses on the function of different AMPs in shrimp responsive to different types of bacteria are very limited. In this study, we analyzed the expression profiles of AMPs in the Chinese shrimps,Fenneropenaeus chinensis, simultaneously by real-time RT-PCR (reverse transcription-polymerase chain reaction) when shrimp were challenged withMicrococcus lysodeikticus(Gram-positive, G+) orVibrio anguillarium(Gram-negative, G−). Different AMPs showed different expression profiles when shrimp were injected with one type of bacterium, and one AMP also showed different expression profiles when shrimp were challenged with different bacteria. Furthermore, the expression of these AMPs showed temporal expression profiles, suggesting that different AMPs function coordinately in bacteria-infected shrimp. An RNA interference approach was used to study the function of the Relish transcription factor in regulating the transcription of different AMPs. The current study showed that Relish could regulate the transcription of different AMPs in shrimp. Differential expression profiles of AMPs in shrimp injected with different types of bacteria indicated that a complicated antimicrobial response network existed in shrimp. These data contribute to our understanding of immunity in shrimp and may provide a strategy for the control of disease in shrimp.
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影响因子：
2.9
作者：
Huang, Xian-De;Yin, Zhi-Xin;He, Jian-Guo
通讯作者：
He, Jian-Guo
DOI：
10.1046/j.1432-1327.1999.00607.x
发表时间：
1999-09-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Relf, JM;Chisholm, JRS;Smith, VJ
通讯作者：
Smith, VJ
影响因子：
1.6
作者：
Li, L;Wang, JX;Kondo, H
通讯作者：
Kondo, H
影响因子：
16
作者：
Hedengren, M;Åsling, B;Hultmark, D
通讯作者：
Hultmark, D
影响因子：
4.1
作者：
Zhang, Jiquan;Li, Fuhua;Xiang, Jianhai
通讯作者：
Xiang, Jianhai