Repression Effects of Hydrolysates from Hen-Egg Proteins on Amyloid Fibril Formation.
Repression Effects of Hydrolysates from Hen-Egg Proteins on Amyloid Fibril Formation.
复制标题
DOI：
10.2141/jpsa.0220038
复制
发表时间：
2022-10-25
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
淀粉样原纤维由异常蛋白质聚集形成，可引起各种形式的淀粉样变性（包括阿尔茨海默病）。此类疾病通常发生在老年人群中，并且被怀疑与生活方式有关。因此，有人推测某些食品可能有益于预防淀粉样变性。在这项研究中，我们通过进行硫黄素 T 测定，然后进行荧光和电子显微镜观察，确定是否可以抑制鸡蛋清溶菌酶 (HEWL) 的原纤维形成。结果表明，鸡蛋清、蛋黄、系带、蛋壳膜粗蛋白水解制备的4种肽标本能有效抑制HEWL原纤维的形成。在这四个样本中，来自合点的肽表现出最高的预防能力。当使用全长人溶菌酶和人淀粉样蛋白 β 肽 1-42 分析原纤维形成时，也观察到了合点肽的优越性，原纤维形成是阿尔茨海默病发展的关键因素。我们对人溶菌酶纤维化的研究还表明，金属离子（Zn2+、Ca2+、Co2+、Mn2+ 和 Al3+）促进纤维化，而肽样本（尤其是合点肽）消除了它们的作用。因此，我们得出结论，鸡蛋蛋白可能是淀粉样变性治疗材料的便利来源。
Amyloid fibrils, which are formed from aggregates of aberrant proteins, can cause various forms of amyloidosis (including Alzheimer's disease). Such disorders often occur in elderly populations and are suspected to be lifestyle related. Thus, it has been speculated that some foodstuffs could be beneficial for preventing amyloidosis. In this study, we determine whether fibril formation by the hen egg white lysozyme (HEWL) could be inhibited by conducting a thioflavin T assay followed by fluorescence and electron microscopy observations. The results demonstrated that four peptide specimens prepared by the hydrolysis of crude proteins from the egg white, egg yolk, chalazae, and eggshell membrane of hen eggs effectively inhibited HEWL fibril formation. Among the four specimens, peptides from chalazae exhibited the highest preventive ability. The superiority of chalaza peptides was also observed when fibril formation was assayed using a full-length human lysozyme and human amyloid β peptide 1–42, which is the key factor for the development of Alzheimer's disease. Our study of the fibrillization of the human lysozyme also showed that metal ions (Zn2+, Ca2+, Co2+, Mn2+ and Al3+) promoted fibrillization, and their effects were abolished by the peptide specimens (especially by chalaza peptides). Thus, we conclude that chicken-egg proteins could be a convenient source of therapeutic materials for amyloidosis.
登录
查看更多内容
影响因子：
3
作者：
Huang, XD;Atwood, CS;Bush, AI
通讯作者：
Bush, AI
DOI：
10.4068/cmj.2014.50.2.45
发表时间：
2014-08
期刊：
Chonnam medical journal
影响因子：
--
作者：
Choi SM;Kim BC;Cho YH;Choi KH;Chang J;Park MS;Kim MK;Cho KH;Kim JK
通讯作者：
Kim JK
DOI：
10.1016/j.biocel.2011.02.009
发表时间：
2011-06-01
影响因子：
4
作者：
Bolognin, Silvia;Messori, Luigi;Zatta, Paolo
通讯作者：
Zatta, Paolo
影响因子：
64.8
作者：
PEPYS, MB;HAWKINS, PN;HSUAN, JJ
通讯作者：
HSUAN, JJ
影响因子：
8.8
作者：
Yang, Kou-Tai;Lin, Chen;Chen, Yi-Chen
通讯作者：
Chen, Yi-Chen