Immunohistochemical Characterization of S100A6 in the Murine Ovary.
Immunohistochemical Characterization of S100A6 in the Murine Ovary.
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DOI：
10.1267/ahc.11035
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发表时间：
2012-02-29
影响因子：
2.4
通讯作者：
Takamatsu K
中科院分区：
文献类型：
--
作者：
Hanaue M;Miwa N;Takamatsu K
S100蛋白包括一个Ca2+结合蛋白大家族，并表现出多种细胞内和细胞外功能。尽管我们对S100蛋白在某些组织（如大脑和平滑肌）中的生物学特性了解得越来越多，但对正常哺乳动物生殖组织中的S100蛋白知之甚少。本研究采用免疫组化方法，利用特异性抗体研究了S100A6（又称calcyclin）在小鼠卵巢中的分布规律。S100A6主要定位于黄体细胞的细胞质中，同时表达另一种S100蛋白S100A11，但不表达于其他类型的细胞，如卵母细胞、卵泡上皮细胞（颗粒细胞）和小鼠卵巢的间质细胞（包括内膜细胞）。s100a6免疫反应性黄体（immunoreactive cora lutea, CLs）分为同质染色和异质染色两种类型，它们可能分别代表分化和成熟的CL。我们的回归分析显示，S100A6的表达水平与细胞色素P450 11A（一种甾体生成酶）的表达水平呈正相关。上述结果提示，S100A6可能与S100A11协同作用，促进黄体细胞甾体生成活性的分化，从而促进某些共享功能。
S100 proteins comprise a large family of Ca2+-binding proteins and exhibit a variety of intra- and extracellular functions. Despite our growing knowledge about the biology of S100 proteins in some tissues such as brain and smooth muscle, little is known about S100 proteins in the normal mammalian reproductive tissue. In the present study, we investigated the distribution pattern of S100A6 (alternatively named calcyclin) in the murine ovary by immunohistochemical study using specific antibody. S100A6 was localized substantially in the cytoplasm of luteal cells, with concomitant expression of S100A11, another S100 protein, but not in the other type of cells such as oocytes, follicle epithelial cells (granulosa cells), and cells of stroma including theca interna cells in the murine ovary. S100A6-immunoreactive corpora lutea (CLs) were divided into two types: homogeneously and heterogeneously stained CLs, and possibly they may represent differentiating and mature CL, respectively. Our regression analysis revealed that expression level of S100A6 positively correlated with that of cytochrome P450 11A, a steroidogenic enzyme in the heterogeously stained CL. These results suggested that S100A6 may contribute to differentiation of steroidogenic activity of luteal cells in a synergistic manner with S100A11 by facilitating some shared functions.
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DOI：
10.1016/0006-291x(65)90320-7
发表时间：
1965-01-01
影响因子：
3.1
作者：
MOORE, BW
通讯作者：
MOORE, BW
DOI：
10.1073/pnas.81.19.6004
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HIRSCHHORN, RR;ALLER, P;BASERGA, R
通讯作者：
BASERGA, R
影响因子：
9.8
作者：
Odegaard, Elin;Davidson, Ben;Staff, Anne Cathrine
通讯作者：
Staff, Anne Cathrine
影响因子：
3.8
作者：
Antonini, JM;Charron, TG;Rogers, RA
通讯作者：
Rogers, RA
影响因子：
6
作者：
Hibbs, K;Skubitz, KM;Skubitz, APN
通讯作者：
Skubitz, APN