Strain-specific changes in the gut microbiota profiles of the white shrimp Litopenaeus vannamei in response to cold stress
Strain-specific changes in the gut microbiota profiles of the white shrimp Litopenaeus vannamei in response to cold stress
复制标题
凡纳滨对虾肠道微生物群特征因冷应激而发生的菌株特异性变化
DOI：
10.1016/j.aquaculture.2019.01.026
复制
发表时间：
2019-03
期刊：
影响因子：
4.5
通讯作者：
Zhang Demin
中科院分区：
文献类型：
--
作者：
Liu Jiajia;Wang Kai;Wang Yanting;Chen Wei;Jin Zhongwen;Yao Zhiyuan;Zhang Demin
肠道微生物群与对虾的生长和健康密切相关。然而，肠道细菌群落对不同对虾品系的环境胁迫（如寒冷）的反应模式及其与宿主状态的关系仍不清楚。本研究采用16S rRNA基因扩增子序列分析技术，对两个白色凡纳滨对虾品系（ZT和PM）的应激耐受性及其与肠道菌群的关系进行了研究。在冷胁迫后（成年期），PM的体重相对稳定，而ZT的体重明显下降，表明PM对冷胁迫具有较强的耐受性。同时，PM肠道细菌的α多样性指数显著高于ZT，PM肠道细菌群落结构也更稳定。此外，冷应激导致主导的细菌组成的ZT肠道的家庭丹毒，支原体科，弧菌科，其中有潜在的致病成员。另一方面，18个歧视性的家庭，代表杜鹃花科（33.8%），在PM肠道的相对丰度显着高于那些在ZT肠道，这表明不同的肠道细菌组合可能反映了虾的抗逆性潜力。耐寒菌株肠道微生物群的分类学特征可以指导从红杆菌科等细菌群中分离新型益生菌，这些益生菌可能用于减少冷应激对白色虾的影响。
The gut microbiota is closely related to shrimp growth and health. However, the response patterns of gut bacterial communities to environmental stresses (such as cold) in distinct shrimp strains and their association with host status are still unclear. In this study, stress tolerance and its potential association with the gut microbiota in two white shrimpLitopenaeus vannameistrains (ZT and PM) were investigated using 16S rRNA gene amplicon sequencing. The stable body weight of PM relative to the obvious weight loss of ZT after both were subjected to cold stress (at the adult life stage) suggests that PM possess stronger tolerance to cold stress. Meanwhile, the α-diversity indices of PM gut bacteria were significantly higher than those of ZT, and the bacterial community structure in the PM gut was more stable as well. Furthermore, cold stress led to domination of the bacterial composition in the ZT gut by the families Erysipelotrichaceae, Mycoplasmataceae, and Vibrionaceae, which have potential pathogenic members. On the other hand, the relative abundances of 18 discriminatory families, represented by Rhodobacteraceae (33.8%), in the PM gut were significantly higher than those in the ZT gut, suggesting that distinct gut bacterial assemblages may reflect the stress resistance potential of shrimp. The taxonomic signature of gut microbiota of the cold-resistant strain could be a guidance for isolation of novel probiotics from bacterial groups such as Rhodobacteraceae, which could be potentially applied to reduce the influence of cold stress in white shrimp.
登录
查看更多内容
影响因子：
24.5
作者：
Schaubeck M;Clavel T;Calasan J;Lagkouvardos I;Haange SB;Jehmlich N;Basic M;Dupont A;Hornef M;von Bergen M;Bleich A;Haller D
通讯作者：
Haller D
虾长大的池塘中的微生物组动力学，可能爆发急性肝癌坏死疾病。
影响因子：
4.6
作者：
Chen WY;Ng TH;Wu JH;Chen JW;Wang HC
通讯作者：
Wang HC
影响因子：
4.5
作者：
Xuxia Zhou;Yanbo Wang;Wei-fen Li
通讯作者：
Xuxia Zhou;Yanbo Wang;Wei-fen Li
DOI：
10.1016/j.jembe.2003.07.002
发表时间：
2003-12
影响因子：
2
作者：
C. Pan;Y. Chien;B. Hunter
通讯作者：
C. Pan;Y. Chien;B. Hunter
影响因子：
3.7
作者：
Son JS;Khair S;Pettet DW 3rd;Ouyang N;Tian X;Zhang Y;Zhu W;Mackenzie GG;Robertson CE;Ir D;Frank DN;Rigas B;Li E
通讯作者：
Li E