QTL mapping for resistance to and tolerance for the rice root-knot nematode, Meloidogyne graminicola.
QTL mapping for resistance to and tolerance for the rice root-knot nematode, Meloidogyne graminicola.
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DOI：
10.1186/s12863-018-0656-1
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发表时间：
2018-08-06
期刊：
影响因子：
2.9
通讯作者：
De Waele D
中科院分区：
文献类型：
--
作者：
Galeng-Lawilao J;Kumar A;De Waele D
禾生根结线虫（Meloidogyne graminicola）是一种专性生物营养型病原体，被认为是对南亚和东南亚的高地和低地水稻生产造成重大产量损失的最具破坏性的线虫物种。利用QTL定位和鉴定鉴定了小麦对白粉病的抗性和耐性。禾生菌可能为农民提供一种安全和经济的管理选择。本研究采用田间试验方法，对小麦对黑胫病的抗性和耐受性进行了研究。亚洲稻（Oryza sativa L.）以300个来自IR 78877 -208-B-1-2的重组自交系（RIL）为作图群体，研究了10个水稻品种的抗病性和耐病性。graminicola，和IR 64，一个受欢迎的高产水稻超级品种，易受M.禾生菌在2012年和2013年旱季对RILs进行了抗性和耐受性表型分析。利用131个单核苷酸多态性（SNP）和33个简单重复序列（SSR）标记进行QTL分析。检测到3个主效QTL（qMGR4.1）、7（qMGR7.1）和9（qMGR9.1），以及2个上位性互作（qMGR3.1/ qMGR11.1和qMGR4.2/ qMGR8.1）。在第4染色体（qGR4.1）和第8染色体（qGR8.1）上发现了一个影响根瘿的QTL，在第5染色体（qYR5.1）和第11染色体（qYR11.1）上发现了一个影响线虫耐性的QTL。这些QTL在两个季节都是一致的。在第10染色体上检测到一个控制产量的QTL（qGYLD10.1），在第11染色体上检测到一个控制每穗实粒数的QTL（qFG11.1），在第2、8、12染色体上分别检测到一个控制根鲜重的QTL（qFRWt2.1、qFRWt8.1、qFRWt12.1）。qMGR 4.1、qMGR 7.1、qMGR 9.1、qGR 4.1、qGR 8.1、qYR 5.1和qFRWt2.1等位基因的供体为IR 78877 -208-B-1-2，而qYR 11.1、qGYLD 10.1和qFG 11.1、qFRWt8.1和qFRWt12.1的供体为IR 64。具有抗性、耐性和产量有利等位基因的品系在线虫侵染条件下具有较好的产量，可作为标记辅助育种（MAB）改良麦长管蚜的起点。亚洲水稻对禾生菌的抗性和耐受性。本研究共检测到12个主效应QTL和2个上位性互作，分别位于第一季和第二季。禾生菌属的抗性和耐受性，以及其它农艺性状，如植物产量、饱满谷粒的百分比以及鲜根和干根重。同时，还筛选出了具有抗性（qMGR4.1、qMGR7.1、qMGR9.1、qGR4.1、qGR8.1）和耐性（qYR5.1和qYR11.1）QTL的有利等位基因的水稻基因型，以及抗性或部分抗性和耐性的基因型。这些筛选的基因型和QTL的定位为设计单抗改良高产但易受稻瘟病影响的基因型提供了重要信息。草生菌感染本文的在线版本（10.1186/s12863-018-0656-1）包含补充材料，可供授权用户使用。
The root-knot nematode Meloidogyne graminicola is an obligate biotrophic pathogen considered to be the most damaging nematode species that causes significant yield losses to upland and rainfed lowland rice production in South and Southeast Asia. Mapping and identification of quantitative trait loci (QTL) for resistance to and tolerance for M. graminicola may offer a safe and economic management option to farmers. In this study, resistance to and tolerance for M. graminicola in Asian rice (Oryza sativa L.) were studied in a mapping population consisting of 300 recombinant inbred lines (RILs) derived from IR78877–208-B-1-2, an aerobic rice genotype with improved resistance to and tolerance for M. graminicola, and IR64, a popular, high-yielding rice mega-variety susceptible to M. graminicola. RILs were phenotyped for resistance and tolerance in the dry seasons of 2012 and 2013. QTL analysis was performed using 131 single nucleotide polymorphism (SNP) and 33 simple sequence repeat (SSR) markers. Three QTLs with main effects on chromosomes 4 (qMGR4.1), 7 (qMGR7.1) and 9 (qMGR9.1) and two epistatic interactions (qMGR3.1/ qMGR11.1 and qMGR4.2/ qMGR8.1) associated with nematode reproduction that were consistent in the two seasons were detected. A QTL affecting root galling was found on chromosomes 4 (qGR4.1) and 8 (qGR8.1), and QTLs for nematode tolerance were found on chromosomes 5 (qYR5.1) and 11 (qYR11.1). These QTLs were consistent in both seasons. A QTL for grain yield was found on chromosome 10 (qGYLD10.1), a QTL affecting filled grains per panicle was detected on chromosome 11 (qFG11.1) and a QTL for fresh root weight was found on chromosomes 2 (qFRWt2.1), 8 (qFRWt8.1) and 12 (qFRWt12.1) in both seasons. The donor of the alleles for qMGR4.1, qMGR7.1, qMGR9.1, qGR4.1, qGR8.1, qYR5.1 and qFRWt2.1 was IR78877–208-B-1-2, whereas for qYR11.1, qGYLD10.1 and qFG11.1, qFRWt8.1 and qFRWt12.1 was IR64. Lines having favorable alleles for resistance, tolerance and yield provided better yield under nematode-infested conditions and could be a starting point of marker-assisted breeding (MAB) for the improvement of M. graminicola resistance and tolerance in Asian rice. This study identified a total of 12 QTLs with main effects and two epistatic interactions in the 1st season and 2nd season related to M. graminicola resistance and tolerance, and other agronomic traits such as plant yield, percentage of filled grains, and fresh and dry root weight. Rice genotypes that have the favorable alleles for resistance (qMGR4.1, qMGR7.1, qMGR9.1, qGR4.1, qGR8.1) and tolerance (qYR5.1, and qYR11.1,) QTLs, and which are either resistant or partially resistant and tolerant, were also selected. These selected genotypes and the identified QTLs are vital information in designing MAB for the improvement of high-yielding rice genotypes but are susceptible to M. graminicola infection. The online version of this article (10.1186/s12863-018-0656-1) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Kumar P;He Y;Singh R;Davis RF;Guo H;Paterson AH;Peterson DG;Shen X;Nichols RL;Chee PW
通讯作者：
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DOI：
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