Rare Cell Capture in Microfluidic Devices.
Rare Cell Capture in Microfluidic Devices.
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DOI：
10.1016/j.ces.2010.09.012
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发表时间：
2011-04-01
影响因子：
4.7
通讯作者：
Kirby BJ
中科院分区：
文献类型：
--
作者：
Pratt ED;Huang C;Hawkins BG;Gleghorn JP;Kirby BJ
本文综述了微流控装置中稀有细胞的分离、分级或捕获方法。稀有细胞捕获装置面临的挑战是维持传统的批量分离方法（如流式细胞仪和免疫磁性分离器）的效率标准，同时需要非常高纯度的靶细胞群，其通常已经处于非常低的起始浓度。稀有细胞捕获方法的两个主要分类包括：（1）非电动方法（例如，通过抗体或适体化学的固定、基于尺寸的分选以及鞘流和流线分选）被讨论用于使用血细胞、癌细胞和其它哺乳动物细胞的应用，以及（2）使用基于电极和绝缘几何形状的电动（主要是介电泳）方法被提出用于病原体检测、血液分级和癌细胞分离。根据性能标准（包括建模和使用的细胞类型、步骤/阶段数、细胞活力和富集、效率和/或纯度）评价所纳入的方法。改进的主要领域是提高直接处理的生物样品的活力和捕获效率/纯度，因为目前大多数研究仅处理加标细胞系或预稀释/裂解样品。尽管目前存在这些挑战，但在稀有细胞捕获装置的开发以及随后对新生物现象的阐明方面取得了多项进展;本文旨在强调这一进展以及电动和非电动方法，这些方法可能会在未来的研究中结合起来以提高性能。
This article reviews existing methods for the isolation, fractionation, or capture of rare cells in microfluidic devices. Rare cell capture devices face the challenge of maintaining the efficiency standard of traditional bulk separation methods such as flow cytometers and immunomagnetic separators while requiring very high purity of the target cell population, which is typically already at very low starting concentrations. Two major classifications of rare cell capture approaches are covered: (1) non-electrokinetic methods (e.g., immobilization via antibody or aptamer chemistry, size-based sorting, and sheath flow and streamline sorting) are discussed for applications using blood cells, cancer cells, and other mammalian cells, and (2) electrokinetic (primarily dielectrophoretic) methods using both electrode-based and insulative geometries are presented with a view towards pathogen detection, blood fractionation, and cancer cell isolation. The included methods were evaluated based on performance criteria including cell type modeled and used, number of steps/stages, cell viability, and enrichment, efficiency, and/or purity. Major areas for improvement are increasing viability and capture efficiency/purity of directly processed biological samples, as a majority of current studies only process spiked cell lines or pre-diluted/lysed samples. Despite these current challenges, multiple advances have been made in the development of devices for rare cell capture and the subsequent elucidation of new biological phenomena; this article serves to highlight this progress as well as the electrokinetic and non-electrokinetic methods that can potentially be combined to improve performance in future studies.
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DOI：
10.1088/0960-1317/16/11/023
发表时间：
2006-11-01
影响因子：
2.3
作者：
Chen, D. F.;Du, H.
通讯作者：
Du, H.
DOI：
10.1073/pnas.92.3.860
发表时间：
1995-01-31
影响因子：
11.1
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
通讯作者：
GASCOYNE, PRC
影响因子：
2.3
作者：
Cui, L;Morgan, H
通讯作者：
Morgan, H
影响因子：
15
作者：
Adams, Andre A.;Okagbare, Paul I.;Soper, Steven A.
通讯作者：
Soper, Steven A.
影响因子：
6.1
作者：
Cheng, I-Fang;Froude, Victoria E.;Chang, Hsien-Chang
通讯作者：
Chang, Hsien-Chang