Hyperaccumulation of arsenic by callus, sporophytes and gametophytes of Pteris vittata cultured in vitro
Hyperaccumulation of arsenic by callus, sporophytes and gametophytes of Pteris vittata cultured in vitro
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体外培养凤尾蕨愈伤组织、孢子体和配子体对砷的超积累
DOI：
10.1007/s00299-007-0388-6
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发表时间：
2007-06
影响因子：
6.2
通讯作者：
中科院分区：
文献类型：
--
作者：
在离体培养基中，由孢子产生的配子体诱导出愈伤组织，愈伤组织生长迅速，培养基周期变化。对拟南芥和维塔塔愈伤组织的砷耐受性和积累量进行了比较。即使砷酸盐浓度达到2 mM，维塔塔愈伤组织也未见细胞死亡；低浓度（0.2 mM）砷酸盐对拟南芥愈伤组织死亡有显著影响。当砷酸盐浓度为0.2 mM时，维塔塔愈伤组织的砷积累量几乎是拟南芥愈伤组织的3倍。7.5 g维塔塔愈伤组织在150 ml含450 μ g砷的半强度MS液体培养基上培养2天后，总砷去除率约为60%。在1 mM砷酸盐处理下，维塔塔愈伤组织、孢子体和配子体均生长良好，累计达1250个；干重1,150和2,180 mg kg(-1)，在2 mM砷酸盐上生长15或30天。维塔草愈伤组织具有细胞未分化、生物量大、易于培养、同步性好、吸附砷效果好等特点，是研究砷污染地下水中砷超富集和植物修复机制的理想系统。
The callus of Pteris vittata was induced from gametophytes generated from spores in vitro, and grew rapidly with periodical medium change. Arsenic tolerance and accumulation of P. vittata callus were compared with those of Arabidopsis thaliana callus. Cell death was not detected in P. vittata callus even at arsenate concentrations up to 2 mM; however, A. thaliana callus died at low (0.2 mM) arsenate concentrations. Meanwhile, P. vittata callus accumulated almost three times more As than A. thaliana callus when exposed to 0.2 mM arsenate. About 60% of the total As was removed when 7.5 g of P. vittata callus was cultured on 150 ml of half-strength MS liquid medium containing 450 microg As for 2 days. Furthermore, P. vittata callus, sporophytes, and gametophytes all grew well under 1 mM of arsenate and accumulated 1,250; 1,150 and 2,180 mg kg(-1 )dry weight As when grown on 2 mM arsenate for 15 or 30 days. The characteristics of non-differentiated cells, large biomass, ease of culture, good synchronization, and excellent As sequestering, make the callus of P. vittata a new ideal system to study the mechanisms of As hyperaccumulation and phytoremediation in As-contaminated groundwater.
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DOI：
10.2136/sssabookser3.3ed
发表时间：
1990
期刊：
--
影响因子：
--
作者：
R. Westerman
通讯作者：
R. Westerman
影响因子：
7.4
作者：
L. Gumaelius;Brett Lahner;D. Salt;J. Banks
通讯作者：
L. Gumaelius;Brett Lahner;D. Salt;J. Banks
影响因子：
8.9
作者：
Weihua Zhang;Yong Cai;K. Downum;L. Ma
通讯作者：
Weihua Zhang;Yong Cai;K. Downum;L. Ma
影响因子：
--
作者：
Chen, TB;Wei, CY;Fan, ZL
通讯作者：
Fan, ZL
影响因子：
9.4
作者：
Lombi, E;Zhao, FJ;McGrath, SP
通讯作者：
McGrath, SP