Genome of a mononucleosis epstein-barr virus contains DNA fragments previously regarded to be unique to Burkitt's lymphoma isolates
Genome of a mononucleosis epstein-barr virus contains DNA fragments previously regarded to be unique to Burkitt's lymphoma isolates
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单核细胞 epstein-barr 病毒的基因组包含以前被认为是伯基特淋巴瘤分离株所独有的 DNA 片段
DOI：
10.1016/0092-8674(81)90345-7
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发表时间：
1981
期刊：
影响因子：
64.5
通讯作者：
W. Summers
中科院分区：
文献类型：
--
作者：
D. K. Fischer;G. Miller;L. Gradoville;L. Heston;Marien W. Weststrate;W. Maris;John Wright;J. Brandsma;W. Summers
我们希望了解从传染性单核细胞增多症患者中分离的EB病毒株的基因组是否与伯基特淋巴瘤株的基因组一致。将从患有单纯性传染性单核细胞增多症的患者的唾液中分离的EBV的新转化株（FF 41）的基因组与Bg 5 -8的DNA进行比较，Bg 5 -8是唯一的其他可从单核细胞增多症获得的病毒。以前已经发现，895-8在由Eco RI C、Hind III D和Barn III片段表示的物理图谱区域中有约8 Md的缺失。来自伯基特淋巴瘤的Wgl和HR-1分离株在该区域中没有缺失，并且已经提出额外的信息是来自伯基特淋巴瘤的EBV分离株的特征。通过限制性内切酶分析、印迹杂交实验和FF 41 DNA的分子克隆，我们证明在Bg 5 -8中发现的缺失在新的单核细胞增多症分离株中不存在。FF 41基因组含有额外的8 Md DNA，由Barn HI片段B ′、W ′和I ′表示，它们位于较大的EcoRI C片段中。因此，这种唾液分离物的基因组含有以前被认为是伯基特淋巴瘤菌株所特有的DNA。因此，EBV基因组中的主要插入或缺失不太可能解释EBV感染后疾病表现的差异。
We wished to learn whether the genomes of strains of EBV isolated from patients with infectious mononucleosis are consistently distinguishable from those of strains from Burkitt’s lymphoma. The genome of a new transforming strain (FF41) of EBV isolated from saliva of a patient with uncomplicated infectious mononucleosis was compared with the DNA of Bg5-8, the only other available virus from mononucleosis. It had been found previously that 895-8 has a deletion of about 8 Md in the region of the physical map represented by the Eco RI C, Hind Ill D, and Barn HI I fragments. The Wgl and HR-1 isolates from Burkitt’s lymphoma are not deleted in this region and it had been proposed that additional information was characteristic of EBV isolates from Burkitt’s lymphoma. By means of restriction enzyme analysis, blot hybridization experiments and molecular cloning of FF41 DNA we demonstrate that the deletion found in Bg5-8 is not present in the new mononucleosis isolate. The, FF41 genome contains an extra 8 Md of DNA, represented by Barn HI fragments B’, W’and I’, which are located in a larger Eco RI C fragment. Thus the genome of this salivary isolate contains DNA that had previously been regarded to be unique to strains from Burkitt’s lymphoma. It is therefore unlikely that major insertions or deletions in the EBV genome account for differences in disease manifestation following EBV infection.
Epstein-Barr 病毒 DNA：对人类胎盘细胞的感染性。
DOI：
10.1126/science.6259735
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Miller,G;Grogan,E;Heston,L;Robinson,J;Smith,D
通讯作者：
Smith,D
对 Epstein-Barr 病毒 B95-8 转化株 DNA 的 BamHi 核酸内切酶片段进行克隆和定位。
DOI：
10.1073/pnas.77.7.3860
发表时间：
1980
影响因子：
11.1
作者：
Skare,J;Strominger,JL
通讯作者：
Strominger,JL