Investigation into mercury bound to biothiols: structural identification using ESI-ion-trap MS and introduction of a method for their HPLC separation with simultaneous detection by ICP-MS and ESI-MS.
Investigation into mercury bound to biothiols: structural identification using ESI-ion-trap MS and introduction of a method for their HPLC separation with simultaneous detection by ICP-MS and ESI-MS.
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DOI：
10.1007/s00216-008-1927-x
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发表时间：
2008-04
影响因子：
4.3
通讯作者：
Feldmann, Jorg
中科院分区：
文献类型：
--
作者：
Krupp, Eva M.;Milne, Bruce F.;Mestrot, Adrien;Meharg, Andrew A.;Feldmann, Jorg
关键词：
植物或动物组织中的汞应该以与生物相关硫醇（生物硫醇）形成的复合物的形式存在，而不是以游离阳离子的形式存在。我们描述了一种分离和分子鉴定汞和甲基汞复合物的技术，这些复合物源自汞和甲基汞与半胱氨酸 (Cys) 和谷胱甘肽 (GS) 的反应：Hg(Cys)2、Hg(GS)2、MeHgCys、MeHgGS。通过配备离子阱的电喷雾质谱 (MS) 对配合物进行了表征，并使用 MP2 和 B3LYP 计算解释了 MeHgCys 的碎片模式，显示了气相中汞-胺相互作用的重要性。使用甲酸作为反相柱上的流动相，在 10 分钟内进行色谱基线分离。通过 ES-MS 和电感耦合等离子体 MS 的在线同步耦合进行检测。当汞络合物添加到真实样品（植物提取物）中时，没有观察到分离和检测条件的干扰，表明该方法能够检测植物中的汞生物硫醇络合物。汞和甲基汞生物硫醇的分离和结构鉴定
Mercury in plants or animal tissue is supposed to occur in the form of complexes formed with biologically relevant thiols (biothiols), rather than as free cation. We describe a technique for the separation and molecular identification of mercury and methylmercury complexes derived from their reactions with cysteine (Cys) and glutathione (GS): Hg(Cys)2, Hg(GS)2, MeHgCys, MeHgGS. Complexes were characterised by electrospray mass spectrometry (MS) equipped with an ion trap and the fragmentation pattern of MeHgCys was explained by using MP2 and B3LYP calculations, showing the importance of mercury–amine interactions in the gas phase. Chromatographic baseline separation was performed within 10 min with formic acid as the mobile phase on a reversed-phase column. Detection was done by online simultaneous coupling of ES-MS and inductively coupled plasma MS. When the mercury complexes were spiked in real samples (plant extracts), no perturbation of the separation and detection conditions was observed, suggesting that this method is capable of detecting mercury biothiol complexes in plants. Separation and structural identification of Hg and MeHg biothiols
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影响因子：
56.9
作者：
Harris, HH;Pickering, IJ;George, GN
通讯作者：
George, GN
DOI：
10.1255/ejms.59
发表时间：
1996-01-01
期刊：
EUROPEAN MASS SPECTROMETRY
影响因子：
--
作者：
DAgostino, A;Colton, R;Canty, AJ
通讯作者：
Canty, AJ
影响因子：
7.4
作者：
Demuth, N;Heumann, KG
通讯作者：
Heumann, KG
DOI：
10.1007/s002160050431
发表时间：
1997-06-01
期刊：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
影响因子：
--
作者：
Hintelmann, H;Falter, R;Evans, RD
通讯作者：
Evans, RD
影响因子：
7.4
作者：
Martín-Doimeadios, RCR;Krupp, E;Donard, OFX
通讯作者：
Donard, OFX