Pseudorabies Viruses from Clinical Outbreaks and Latent Infections Grouped into Four Major Genome Types
Pseudorabies Viruses from Clinical Outbreaks and Latent Infections Grouped into Four Major Genome Types
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临床暴发和潜伏感染的伪狂犬病病毒分为四种主要基因组类型
DOI：
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
H. Ludwig
中科院分区：
文献类型：
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作者：
S. Herrmann;B. Heppner;H. Ludwig
从世界各地的猪、牛和食肉动物中收集了大约150株伪狂犬病病毒。用限制性内切酶分析了它们的DNA，并与已发表的标准菌株的物理图谱进行了比较。此外，调查中还包括4种疫苗株。四个主要的基因组组是明显的，聚集在不同的地理区域（北方，中欧和泰国）。这些基因组组内的分离株还可以根据动物流行病学相关性进行细分。通过杂交确定来自不同组的DNA的核苷酸序列同源性。四种疫苗株的DNA模式与野生型菌株的DNA模式略有不同，有证据表明其中两种疫苗株在基因组的短独特区域中存在缺失。
About 150 isolates of pseudorabies virus were collected worldwide from fatally infected piglets, cattle and carnivores. Their DNAs were analysed by restriction enzmye cleavage and compared with the published physical map of a standard strain. In addition, four vaccine strains were included in the investigation. Four main genome groups were evident, clustering in distinct geographic areas (Northern Europe, Middle Europe and Thailand). Isolates within these genome groups can additionally be subdivided according to epizootiological relatedness. Nucleotide sequence homology of the DNAs derived from the different groups was ascertained by hybridization. The DNA patterns of the four vaccine strains are slightly different from those of wildtype strains and there is evidence that two of the vaccine strains have a deletion in the short unique region of the genome.
通过限制性核酸内切酶区分马疱疹病毒 1 的呼吸道和致流产分离株。
DOI：
10.1126/science.6270790
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
Studdert,MJ;Simpson,T;Roizman,B
通讯作者：
Roizman,B