Restoration of miR-1228* expression suppresses epithelial-mesenchymal transition in gastric cancer.
Restoration of miR-1228* expression suppresses epithelial-mesenchymal transition in gastric cancer.
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DOI：
10.1371/journal.pone.0058637
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cai J
中科院分区：
文献类型：
--
作者：
Jia L;Wu J;Zhang L;Chen J;Zhong D;Xu S;Xie C;Cai J
失调的mirna在癌症发生和癌症进展中起着关键作用。本研究探讨miR-1228*在胃癌中调控肿瘤进展的功能。与正常组织相比，miR-1228*在人胃癌组织中表达降低。随后，使用肿瘤异种移植模型在体内评估miR-1228*的作用。在该模型中，miR-1228*过表达抑制异种移植瘤的形成。此外，我们证实了miR-1228*在SGC-7901胃癌细胞中负调控NF-κB活性，并发现CK2A2是miR-1228*的靶点。miR-1228*的上调降低了间质标志物的表达，增加了上皮标志物E-cadherin的表达，提示其在抑制上皮-间质转化中的潜在作用。综上所述，这些发现首次证明miR-1228*在调节胃癌生长中发挥重要作用，并提示选择性恢复miR-1228*可能有益于胃癌治疗。
Dysregulated miRNAs play critical roles during carcinogenesis and cancer progression. In the present study, the function of miR-1228* in regulating cancer progression was investigated in gastric cancer. Decreased expression of miR-1228* was observed in human gastric cancer tissues comparing to normal tissues. Subsequently, the role of miR-1228* was evaluated in vivo using the tumor xenograft model. In this model, miR-1228* overexpression suppressed xenograft tumor formation. Furthermore, we demonstrated miR-1228* negatively regulated NF-κB activity in SGC-7901 gastric cancer cells and found that CK2A2 was a target of miR-1228*. Upregulation of miR-1228* decreased the expression of mesenchymal markers and increased the epithelial marker E-cadherin, suggesting its potential role in suppressing epithelial-mesenchymal transition. Collectively, these findings provide the first evidence that miR-1228* plays an important role in regulating gastric cancer growth and suggest that selective restoration of miR-1228* might be beneficial for gastric cancer therapy.
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