Assessment of DNA damage and repair in adults consuming allyl isothiocyanate or Brassica vegetables.
Assessment of DNA damage and repair in adults consuming allyl isothiocyanate or Brassica vegetables.
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DOI：
10.1016/j.jnutbio.2012.06.004
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Novotny JA
中科院分区：
文献类型：
--
作者：
Charron CS;Clevidence BA;Albaugh GA;Kramer MH;Vinyard BT;Milner JA;Novotny JA
异硫氰酸烯丙酯（AITC）是一种可能具有抗癌作用的膳食成分，尽管已经从细胞研究中获得了许多关于AITC和癌症的信息。为了研究AITC对体内DNA完整性的影响，进行了交叉研究。成年人（n = 46）食用AITC，富含AITC的蔬菜（芥菜和卷心菜），或对照治疗与控制饮食各10天。在第11天，志愿者在服用治疗前后提供血液和尿液。对志愿者进行了GSTM 1和GSTT1（谷胱甘肽S-转移酶）和XPD（DNA修复）基因型的表征。通过单细胞凝胶电泳评估外周血单个核细胞（PBMC）中的DNA完整性。分析尿液中的8-氧代-7，8-二氢-2'-脱氧鸟苷（8-oxodG）和肌酐。10天摄入AITC或芥菜/卷心菜（M/C）均未导致DNA链断裂的统计学显著差异（尾部中的LS平均%DNA ± SEM：对照组为4.8 ± 0.6，AITC为5.7 ± 0.7，M/C为5.3 ± 0.6）或尿8-oxodG（LS平均值µ g 8-oxodG/g肌酐± SEM：对照组为2.95 ± 0.09，AITC为2.88 ± 0.09，M/C为3.06 ± 0.09）。AITC和M/C均增加了食用后3小时的DNA链断裂（尾部中的LS平均%DNA ± SEM：对照组为3.2 ± 0.7，AITC为8.3 ± 1.7，M/C为8.0 ± 1.7），并且这种差异在6小时消失（对照组为4.2 ± 0.9，AITC为5.7 ± 1.2，M/C为5.5 ± 1.2）。GSTM1、GSTT1和XPD的基因型与治疗效果无关。总之，AITC和富含AITC的产物似乎在短期内诱导DNA损伤，但这种损伤很快消失，AITC和富含AITC的产物都不影响DNA碱基切除修复。
Allylisothiocyanate (AITC) is a dietary component with possible anti-cancer effects, though much information about AITC and cancer has been obtained from cell studies. To investigate the effect of AITC on DNA integrity in vivo, a crossover study was conducted. Adults (n=46) consumed AITC, AITC-rich vegetables (mustard and cabbage), or a control treatment with a controlled diet for 10 days each. On day 11, volunteers provided blood and urine before and after consuming treatments. Volunteers were characterized for genotype for GSTM1 and GSTT1 (glutathione S-transferases) and XPD (DNA repair). DNA integrity in peripheral blood mononuclear cells (PBMCs) was assessed by single cell gel electrophoresis. Urine was analyzed for 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodG) and creatinine. Ten day intake of neither AITC nor mustard/cabbage(M/C) resulted in statistically significant differences in DNA strand breaks (LS mean % DNA in tail ± SEM: 4.8 ± 0.6 for control, 5.7 ± 0.7 for AITC, 5.3 ± 0.6 for M/C) or urinary 8-oxodG (LS mean µg 8-oxodG/g creatinine ± SEM: 2.95 ± 0.09 for control, 2.88 ± 0.09 for AITC, 3.06 ± 0.09 for M/C). Both AITC and M/C increased DNA strand breaks 3h post-consumption (LS mean % DNA in tail ± SEM: 3.2 ± 0.7 for control, 8.3 ± 1.7 for AITC, 8.0 ± 1.7 for M/C), and this difference disappeared at 6h (4.2 ± 0.9 for control, 5.7 ± 1.2 for AITC, 5.5 ± 1.2 for M/C). Genotypes for GSTM1, GSTT1, and XPD were not associated with treatment effects. In summary, DNA damage appeared to be induced in the short term by AITC and AITC-rich products, but that damage disappeared quickly, and neither AITC nor AITC-rich products affected DNA base excision repair.
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