High-quality human preimplantation embryos actively influence endometrial stromal cell migration.
High-quality human preimplantation embryos actively influence endometrial stromal cell migration.
复制标题
DOI：
10.1007/s10815-017-1107-z
复制
发表时间：
2018-04
影响因子：
3.1
通讯作者：
Mastenbroek S
中科院分区：
文献类型：
--
作者：
Berkhout RP;Lambalk CB;Huirne J;Mijatovic V;Repping S;Hamer G;Mastenbroek S
本文的目的是研究人类着床前胚胎是否调节子宫内膜基质细胞（hESC）的迁移。原代hESCs从良性子宫切除术（子宫瘢痕生态位n = 3，痛经n = 2，未接受激素治疗）的有生育能力患者中分离出来。在高质量胚胎（破碎率≤20%）或低质量胚胎（破碎率≤20%）的胚胎条件培养基（ECM）中，或在来自相同培养皿的非条件培养基（对照）中培养脱去或非脱去的hESCs，进行迁移和增殖试验。本研究使用了来自425个单独培养的人类胚胎的ECM样本。来自高质量胚胎的ECM，即低碎片率，积极刺激去个体化hESC迁移（p < 0.001）。这种影响在胚胎发育过程中是一致的，从2-7个细胞的卵裂期胚胎（高质量与对照组相比，p = 0.036）、8-18个细胞的胚胎（高质量与对照组相比，p < 0.001）到桑葚胚（高质量与对照组相比，p = 0.003）。线性回归分析表明，胚胎质量（破碎度，β - 0.299, p = 0.025）影响hESC迁移，而发育阶段（细胞数，β - 0.177, p = 0.176）和母亲年龄（β - 0.036, p = 0.78）不影响hESC迁移。与去细胞化hESCs相反，非去细胞化hESCs的迁移反应受到优质胚ECM的抑制（p = 0.019）。来自低质量胚胎的ECM，即高破碎率的ECM，在蜕质hESCs （p = 0.860）或非蜕质hESCs （p = 0.986）中没有引起迁移反应的改变。此外，高质量和低质量人胚胎的ECM均不影响非脱体细胞和脱体细胞hESCs的增殖细胞数量（p = 0.375）和细胞周期时间（p = 0.297）。本研究揭示了高质量的人类着床前胚胎与子宫内膜积极相互作用以增加其成功着床机会的机制。本文的在线版本（10.1007/s10815-017-1107-z）包含补充内容，仅供授权用户使用。
The purpose of this paper is to study whether human preimplantation embryos regulate endometrial stromal cell (hESC) migration. Primary hESCs were isolated from fertile patients undergoing hysterectomy for benign conditions (uterine scar niche n = 3, dysmenorrhea n = 2; no hormonal treatment). Migration and proliferation assays were performed by culturing decidualized or non-decidualized hESCs in the presence of embryo conditioned medium (ECM) from high-quality embryos (fragmentation ≤ 20%) or from low-quality embryos (fragmentation > 20%) or in non-conditioned medium from the same dishes (control). ECM samples from 425 individually cultured human embryos were used in this study. ECM from high-quality embryos, i.e., with a low percentage of fragmentation, actively stimulated decidualized hESC migration (p < 0.001). This effect was consistent throughout embryonic development from cleavage stage embryos with 2–7 cells (high quality vs. control; p = 0.036), 8–18 cells (high quality vs. control; p < 0.001) to morulae (high quality vs. control; p = 0.003). Additionally, linear regression analysis showed that hESC migration was influenced by embryo quality (fragmentation, β − 0.299; p = 0.025) and not developmental stage (cell number, β 0.177; p = 0.176) or maternal age (β − 0.036; p = 0.78). Opposite to decidualized hESCs, the migration response of non-decidualized hESCs was inhibited by ECM from high-quality embryos (p = 0.019). ECM from low-quality embryos, i.e., with a high percentage of fragmentation, did not cause an altered migration response in decidualized hESCs (p = 0.860) or non-decidualized hESCs (p = 0.986). Furthermore, ECM of both high- and low-quality human embryos did not influence the number of proliferating cells (p = 0.375) and the cell cycle time (p = 0.297) of non-decidualized or decidualized hESCs. This study reveals a mechanism by which high-quality human preimplantation embryos actively interact with the endometrium to increase their chances of successful implantation. The online version of this article (10.1007/s10815-017-1107-z) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
82.9
作者：
Cha J;Sun X;Dey SK
通讯作者：
Dey SK
影响因子：
3.7
作者：
Teklenburg G;Salker M;Molokhia M;Lavery S;Trew G;Aojanepong T;Mardon HJ;Lokugamage AU;Rai R;Landles C;Roelen BA;Quenby S;Kuijk EW;Kavelaars A;Heijnen CJ;Regan L;Brosens JJ;Macklon NS
通讯作者：
Macklon NS
影响因子：
158.5
作者：
Malizia, Beth A.;Hacker, Michele R.;Penzias, Alan S.
通讯作者：
Penzias, Alan S.
影响因子：
3.6
作者：
Macklon, Nick S;Brosens, Jan J
通讯作者：
Brosens, Jan J
DOI：
10.1073/pnas.0806219105
发表时间：
2008-10-21
影响因子：
11.1
作者：
Grewal, Seema;Carver, Janet G.;Mardon, Helen J.
通讯作者：
Mardon, Helen J.