Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum.
Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum.
复制标题
DOI：
10.1371/journal.pone.0126883
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Edwards RA
中科院分区：
文献类型：
--
作者：
Matthews TD;Schmieder R;Silva GG;Busch J;Cassman N;Dutilh BE;Green D;Matlock B;Heffernan B;Olsen GJ;Farris Hanna L;Schifferli DM;Maloy S;Dinsdale EA;Edwards RA
肠道沙门氏菌血清型肠沙门氏菌、都柏林沙门氏菌和鸡沙门氏菌密切相关，但在毒力和宿主范围上不同。为了鉴定导致这些差异的遗传元件并更好地理解这些血清型是如何进化的，我们对肠杆菌菌株LK 5和都柏林菌株SARB 12的基因组进行了测序，并将这些基因组与公开获得的肠杆菌P125109、都柏林CT 02021853和都柏林SD 3246基因组序列进行了比较。我们还比较了来自生物型鸡287/91和鸡白痢RKS 5078的公开可用的鸡基因组序列。使用生物信息学方法，我们确定了单核苷酸多态性，插入，缺失，以及属于同一血清型的菌株之间的原噬菌体和假基因含量的差异。通过我们的分析，我们还确定了几个前噬菌体货物基因和假基因，影响毒力，并可能有助于主机特定的，系统的生活方式。这些结果有力地证明了肠道沙门氏菌的肠杆菌、都柏林和鸡血清型是通过水平基因转移获得新基因，然后形成假基因而进化的。胃肠道生活方式所必需的基因的丢失最终导致宿主特异性血清型中的系统性生活方式和生态位排斥。
The Salmonella enterica serovars Enteritidis, Dublin, and Gallinarum are closely related but differ in virulence and host range. To identify the genetic elements responsible for these differences and to better understand how these serovars are evolving, we sequenced the genomes of Enteritidis strain LK5 and Dublin strain SARB12 and compared these genomes to the publicly available Enteritidis P125109, Dublin CT 02021853 and Dublin SD3246 genome sequences. We also compared the publicly available Gallinarum genome sequences from biotype Gallinarum 287/91 and Pullorum RKS5078. Using bioinformatic approaches, we identified single nucleotide polymorphisms, insertions, deletions, and differences in prophage and pseudogene content between strains belonging to the same serovar. Through our analysis we also identified several prophage cargo genes and pseudogenes that affect virulence and may contribute to a host-specific, systemic lifestyle. These results strongly argue that the Enteritidis, Dublin and Gallinarum serovars of Salmonella enterica evolve by acquiring new genes through horizontal gene transfer, followed by the formation of pseudogenes. The loss of genes necessary for a gastrointestinal lifestyle ultimately leads to a systemic lifestyle and niche exclusion in the host-specific serovars.
登录
查看更多内容
DOI：
10.2174/1874285801206010005
发表时间：
2012
期刊：
The open microbiology journal
影响因子：
--
作者：
Betancor L;Yim L;Martínez A;Fookes M;Sasias S;Schelotto F;Thomson N;Maskell D;Chabalgoity JA
通讯作者：
Chabalgoity JA
影响因子：
4.2
作者：
Betancor L;Yim L;Fookes M;Martinez A;Thomson NR;Ivens A;Peters S;Bryant C;Algorta G;Kariuki S;Schelotto F;Maskell D;Dougan G;Chabalgoity JA
通讯作者：
Chabalgoity JA
影响因子：
6.4
作者：
Desai PT;Porwollik S;Long F;Cheng P;Wollam A;Bhonagiri-Palsikar V;Hallsworth-Pepin K;Clifton SW;Weinstock GM;McClelland M
通讯作者：
McClelland M
影响因子：
3.6
作者：
Figueroa-Bossi, N;Uzzau, S;Bossi, L
通讯作者：
Bossi, L
影响因子：
1.6
作者：
FANG, FC;FIERER, J
通讯作者：
FIERER, J