Faecal tumour M2 pyruvate kinase: a new, sensitive screening tool for colorectal cancer.
Faecal tumour M2 pyruvate kinase: a new, sensitive screening tool for colorectal cancer.
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DOI：
10.1038/sj.bjc.6602033
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发表时间：
2004-08-31
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
肿瘤细胞，特别是肿瘤细胞，表达一种特殊的丙酮酸激酶同工酶，称为M2-PK，它可以以对其底物磷酸烯醇丙酮酸（PEP）具有高亲和力的四聚体形式存在，也可以以具有低PEP亲和力的二聚体形式存在。在肿瘤细胞中，二聚体形式通常占主导地位，因此称为肿瘤M2-PK。肿瘤内和EDTA-血浆中的肿瘤M2-PK水平与肿瘤细胞的分期和转移能力相关。由于大多数结直肠肿瘤在腔内生长，因此确定肿瘤患者粪便中是否可检测到肿瘤M2-PK似乎很有趣。通过ELISA检测了未患结直肠癌的对照组和结直肠癌患者的粪便样本。而对照组的肿瘤M2-PK水平较低（平均值±s.e.m.：3.3±0.4，n=144），大肠癌患者中较高（56.1±15.3，n=60）。当临界值为4 U ml−1时，灵敏度为73%。肿瘤的TNM和Dukes分类揭示了粪便肿瘤M2-PK水平和分期之间的强相关性。粪便中肿瘤M2-PK的测定为结直肠肿瘤提供了一种新的有前途的筛查工具。
Proliferating cells, especially tumour cells, express a special isoenzyme of pyruvate kinase, termed M2-PK, which can occur in a tetrameric form with a high affinity to its substrate, phosphoenolpyruvate (PEP), and in a dimeric form with a low PEP affinity. In tumour cells, the dimeric form is usually predominant and is therefore termed Tumour M2-PK. The levels of Tumour M2-PK within tumours and in EDTA-plasma correlate with staging and the ability of the tumour cells to metastasise. Since most colorectal tumours grow intraluminally, it appeared interesting to determine whether Tumour M2-PK is detectable in the faeces of tumour patients. Stool samples were tested by ELISA from controls without colorectal cancer and colorectal cancer patients. Whereas Tumour M2-PK levels were low in the control group (mean value±s.e.m.: 3.3±0.4, n=144), they were high in the case of colorectal cancer (56.1±15.3, n=60). At a cutoff value of 4 U ml−1, the sensitivity was 73%. TNM and Dukes' classification of the tumours revealed a strong correlation between faecal Tumour M2-PK levels and staging. The determination of Tumour M2-PK in faeces provides a new promising screening tool for colorectal tumours.
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影响因子：
4.8
作者：
Boren, J;Lee, WNP;Cascante, M
通讯作者：
Cascante, M
影响因子：
29.4
作者：
Ahlquist, DA;Skoletsky, JE;Shuber, AP
通讯作者：
Shuber, AP
DOI：
10.1016/s0065-2571(01)00036-x
发表时间：
2002-01-01
期刊：
ADVANCES IN ENZYME REGULATION, VOL 42, PROCEEDINGS
影响因子：
--
作者：
Le Mellay, V;Houben, R;Rapp, UR
通讯作者：
Rapp, UR
DOI：
10.1073/pnas.84.8.2251
发表时间：
1987-04-01
影响因子：
11.1
作者：
BOLEN, JB;VEILLETTE, A;ROSEN, N
通讯作者：
ROSEN, N
DOI：
10.1093/jnci/93.11.858
发表时间：
2001-06-06
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Dong, SM;Traverso, G;Jen, J
通讯作者：
Jen, J