A Chinese Herbal Formula Ameliorates Pulmonary Fibrosis by Inhibiting Oxidative Stress via Upregulating Nrf2.
A Chinese Herbal Formula Ameliorates Pulmonary Fibrosis by Inhibiting Oxidative Stress via Upregulating Nrf2.
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中草药配方通过上调 Nrf2 抑制氧化应激来改善肺纤维化
DOI：
10.3389/fphar.2018.00628
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhou T
中科院分区：
文献类型：
--
作者：
Bai Y;Li J;Zhao P;Li Y;Li M;Feng S;Qin Y;Tian Y;Zhou T
This study aimed to explore the protective effects of a Chinese herbal formula, Jinshui Huanxian formula (JHF), on experimental pulmonary fibrosis and its underlying mechanisms. After being treated with single dose of bleomycin (5 mg/kg) intratracheally, rats were orally administered with JHF and pirfenidone from day 1 to 42, then sacrificed at 7, 14, 28, or 42 days post-bleomycin instillation. JHF ameliorated bleomycin-induced pathological changes, collagen deposition in the rat lung and recovered pulmonary function at different days post-bleomycin instillation. In lungs of JHF-treated rats, the levels of total superoxide dismutase, catalase and glutathione were higher, and myeloperoxidase and methane dicarboxylic aldehyde were lower than those in vehicle-treated rats, respectively. Additionally, JHF inhibited the expression of NADPH oxidase 4 (NOX4) and increased the Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) in lung tissues. In vitro, JHF and ruscogenin, a compound of Ophiopogonis Radix contained in JHF, significantly inhibited transforming growth factor β1 (TGF-β1)-induced differentiation of fibroblasts. Furthermore, JHF markedly decreased the level of reactive oxygen species in TGF-β1-induced fibroblast. In line with this, upregulation of NAD(P)H: quinone oxidoreductase 1 and heme oxygenase 1, and downregulation of NOX4 were found in JHF-treated fibroblast induced by TGF-β1. While on the other hand, Nrf2 siRNA could suppress the JHF-mediated inhibition effect on alpha-smooth muscle actin (α-SMA), and FN1 expression induced by TGF-β1 in fibroblasts. These results indicated that JHF performed remarkably therapeutic and long-term effects on pulmonary fibrosis in rat and suppressed the differentiation of fibroblast into myofibroblast through reducing the oxidative response by upregulating Nrf2 signaling. It might provide a new potential natural drug for the treatment of pulmonary fibrosis.
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痤疮丙酸杆菌加强攻击诱导结节肉芽肿小鼠模型的肺纤维化
影响因子：
--
作者：
Jiang D;Huang X;Geng J;Dong R;Li S;Liu Z;Wang C;Dai H
通讯作者：
Dai H
DOI：
10.1165/rcmb.2013-0174oc
发表时间：
2014-01-01
影响因子：
6.4
作者：
Jarman, Elizabeth R.;Khambata, Valerie S.;Jarai, Gabor
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Jarai, Gabor
影响因子：
3.2
作者：
Daniil, Zoe D.;Papageorgiou, Evangelia;Gourgoulianis, Konstantinos I.
通讯作者：
Gourgoulianis, Konstantinos I.
DOI：
10.1164/ajrccm.162.1.9903129
发表时间：
2000-07-01
影响因子：
24.7
作者：
Hagiwara, S;Ishii, Y;Kitamura, S
通讯作者：
Kitamura, S
影响因子：
7.4
作者：
Liu, Rui-Ming;Vayalil, Praveen Kumar;Ballinger, Carol;Dickinson, Dale A.;Huang, Wen-Tan;Wang, Suqing;Kavanagh, Terrance J.;Matthews, Qiana L.;Postlethwait, Edward M.
通讯作者：
Postlethwait, Edward M.