Origin of neuropeptide Y-containing afferents to gonadotropin-releasing hormone neurons in male mice.
Origin of neuropeptide Y-containing afferents to gonadotropin-releasing hormone neurons in male mice.
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雄性小鼠促性腺激素释放激素神经元的含神经肽 Y 传入的起源。
DOI：
10.1210/en.2003-0470
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发表时间：
2003
期刊：
影响因子：
4.8
通讯作者：
E. Hrabovszky
中科院分区：
文献类型：
--
作者：
G. Turi;Zsolt Liposits;S. Moenter;C. Fekete;E. Hrabovszky
The origin of neuropeptide Y (NPY) afferents to GnRH neurons was investigated in male mice. Neonatal lesioning of the hypothalamic arcuate nuclei (ARC) with monosodium glutamate markedly reduced the number of NPY fibers in the preoptic area as well as the frequency of their contacts with perikarya and proximal dendrites of GnRH neurons. Dual-label immunofluorescence studies to determine the precise contribution of the ARC to the innervation of GnRH neurons by NPY axons were carried out on transgenic mice in which enhanced green fluorescent protein was expressed under the control of the GnRH promoter (GnRH-enhanced green fluorescent protein mice). The combined application of red Cy3 and blue AMCA fluorochromogenes established that 49.1 +/- 7.3% of NPY axons apposed to green GnRH neurons also contained agouti-related protein (AGRP), a selective marker for NPY axons arising from the ARC. Immunoelectronmicroscopic analysis detected symmetric synapses between AGRP fibers and GnRH-immunoreactive perikarya. Additional triple-fluorescence experiments revealed the presence of dopamine-beta-hydroxylase immunoreactivity within 25.4 +/- 3.3% of NPY afferents to GnRH neurons. This enzyme marker enabled the selective labeling of NPY pathways ascending from noradrenergic/adrenergic cell populations of the brain stem, thus defining a second important source for NPY-containing fibers regulating GnRH cells. The absence of both topographic markers (AGRP and dopamine-beta-hydroxylase) within 26% of NPY contacts suggests that additional sources of NPY fibers to GnRH neurons exist. Future studies will address distinct functions of the two identified NPY systems in the afferent neuronal regulation of the GnRH system.
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DOI：
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发表时间：
--
期刊：
--
影响因子：
--
作者：
通讯作者：
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影响因子：
7.7
作者：
Baskin, DG;Breininger, JF;Schwartz, MW
通讯作者：
Schwartz, MW
神经肽 Y 诱导雄性大鼠正中隆起片段释放黄体生成素释放激素的类固醇调节。
影响因子：
4.1
作者：
Urban,JH;Das,I;Levine,JE
通讯作者：
Levine,JE
影响因子：
4.8
作者：
K. Mounzih;R. Lu;Farid F. Chehab
通讯作者：
K. Mounzih;R. Lu;Farid F. Chehab
仅在导致排卵前 LH 激增的情况下，神经肽 Y 才会增强黄体生成素 (LH) 释放激素诱导的 LH 分泌。
影响因子：
4.8
作者：
Bauer-Dantoin,AC;McDonald,JK;Levine,JE
通讯作者：
Levine,JE