Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain
Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain
复制标题
fos 和 jun 核癌蛋白之间的直接相互作用：“亮氨酸拉链”结构域的作用
作者：
P. Sassone;L. Ransone;W. Lamph;I. Verma
基因表达是通过核蛋白与基因组中独特的调控序列的特定相互作用来调节的。参与转录调节的蛋白质似乎是转录因子或转录调节剂，它们的相互作用对于确定特定基因的表达是激活还是抑制至关重要。最近 1，原癌基因 jun 的产物被鉴定为转录因子 AP-1，而核癌蛋白 fos 和 myc 与多个启动子的转录反式调节有关2-6。此外，fos 和 jun 原癌基因的产物与一些转录复合物相关3,5,7。尽管这种关联的性质尚不清楚，但这两种蛋白与核提取物中的 fos 抗体发生共免疫沉淀8-10。在这里，我们报告的研究表明 fos 蛋白通过 fos 和 jun 蛋白的异二聚体直接调节 jun 功能。 fos“亮氨酸拉链”11 结构域对于异源二聚体的 DNA 结合是必需的；位于 fos 蛋白 C 末端区域的一个独特结构域负责转录调节。
Gene expression is modulated by the specific interactions of nuclear proteins with unique regulatory sequences in the genome. Proteins involved in transcriptional regulation seem to be either transcription factors or transcription modulators and their interactions are crucial in determining whether the expression of a specific gene is activated or repressed. Recently1, the product of the proto-oncogene jun has been identified as the transcription factor AP-1, whereas nuclear oncoproteins fos and myc have been implicated in transcriptional transregulation of several promoters2–6. Furthermore, the products of the fos and jun proto-oncogenes are associated in some transcription complexes3,5,7. Although the nature of the association is unclear, the two proteins co-immunoprecipitate with fos antibodies in nuclear extracts8–10. Here, we report studies that demonstrate that the fos protein directly modulates jun function by means of a heterodimer of fos and jun proteins. The fos 'leucine zipper'11 domain is necessary for the DNA binding of the heterodimer; a distinct domain, localized in the C-terminal region of the fos protein, is responsible for transcriptional regulation.
影响因子：
56.9
作者：
RAUSCHER, FJ;COHEN, DR;FRANZA, BR
通讯作者：
FRANZA, BR
影响因子：
56.9
作者：
BOHMANN, D;BOS, TJ;TJIAN, R
通讯作者：
TJIAN, R