A methodology to establish a database to study gene environment interactions for childhood asthma.
A methodology to establish a database to study gene environment interactions for childhood asthma.
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DOI：
10.1186/1471-2288-10-107
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发表时间：
2010-12-06
影响因子：
4
通讯作者：
Macgregor D
中科院分区：
文献类型：
--
作者：
Turner SW;Ayres JG;Macfarlane TV;Mehta A;Mehta G;Palmer CN;Cunningham S;Adams T;Aniruddhan K;Bell C;Corrigan D;Cunningham J;Duncan A;Hunt G;Leece R;MacFadyen U;McCormick J;McLeish S;Mitra A;Miller D;Waxman E;Webb A;Wojcik S;Mukhopadhyay S;Macgregor D
基因-环境相互作用可能解释儿童哮喘的某些异质性。在这里，我们描述的方法和经验，在建立一个数据库的儿童哮喘，旨在研究基因环境的相互作用（PAGES -儿科哮喘基因环境研究）。2008年至2011年期间，从15家医院招募了接受呼吸儿科医生护理的哮喘儿童。填写哮喘调查问卷并邮寄。在常规的诊所访问中，收集唾液用于DNA提取。一部分儿童的详细表型包括肺量测定、支气管扩张反应（BDR）、皮肤点刺反应、呼出一氧化氮和唾液可替宁。完成饮食和生活质量问卷。数据被输入到一个专门建立的数据库。迄今为止，已邀请1 045名儿童参加，并收集了501名儿童（48%）的数据。参与者的平均年龄（SD）为8.6（3.9）岁，57%为男性。已收集了436名儿童的DNA。对172名儿童进行了肺功能测定，平均%预测（SD）FEV 1 97%（15），中位数（IQR）BDR为5%（2，9）。不同中心之间的年龄、社会经济状况、严重程度和%FEV1存在差异（p≤0.024）。未参与的原因包括父母没有时间参与，儿童没有到诊所就诊，以及少数儿童拒绝参与。建立一个全国性的数据库来研究哮喘儿童人群中的基因-环境相互作用是可行的;从不同中心招募的个体存在参与障碍和一些不同的特征。本研究的招募仍在继续，预计将扩大目前对哮喘异质性的理解。
Gene-environment interactions are likely to explain some of the heterogeneity in childhood asthma. Here, we describe the methodology and experiences in establishing a database for childhood asthma designed to study gene-environment interactions (PAGES - Paediatric Asthma Gene Environment Study). Children with asthma and under the care of a respiratory paediatrician are being recruited from 15 hospitals between 2008 and 2011. An asthma questionnaire is completed and returned by post. At a routine clinic visit saliva is collected for DNA extraction. Detailed phenotyping in a proportion of children includes spirometry, bronchodilator response (BDR), skin prick reactivity, exhaled nitric oxide and salivary cotinine. Dietary and quality of life questionnaires are completed. Data are entered onto a purpose-built database. To date 1045 children have been invited to participate and data collected in 501 (48%). The mean age (SD) of participants is 8.6 (3.9) years, 57% male. DNA has been collected in 436 children. Spirometry has been obtained in 172 children, mean % predicted (SD) FEV1 97% (15) and median (IQR) BDR is 5% (2, 9). There were differences in age, socioeconomic status, severity and %FEV1 between the different centres (p≤0.024). Reasons for non-participation included parents not having time to take part, children not attending clinics and, in a small proportion, refusal to take part. It is feasible to establish a national database to study gene-environment interactions within an asthmatic paediatric population; there are barriers to participation and some different characteristics in individuals recruited from different centres. Recruitment to our study continues and is anticipated to extend current understanding of asthma heterogeneity.
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DOI：
10.1177/003591577206500320
发表时间：
1972-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY OF MEDICINE-LONDON
影响因子：
--
作者：
PEPYS, J
通讯作者：
PEPYS, J
影响因子：
5
作者：
Botto, LD;Khoury, MJ
通讯作者：
Khoury, MJ
影响因子：
5
作者：
HWANG, SJ;BEATY, TH;KHOURY, MJ
通讯作者：
KHOURY, MJ
DOI：
10.1164/rccm.200406-710st
发表时间：
2005-04-15
影响因子：
24.7
作者：
American Thoracic Society;European Respiratory Society
通讯作者：
European Respiratory Society
影响因子：
5.2
作者：
McCormick, J;Green, MW;Mehta, A
通讯作者：
Mehta, A